PdeH

• Description: c-di-GMP degrading phosphodiesterase
  
  

Gene name	yuxH
Synonyms	comB, yufA
Essential	no
Product	c-di-GMP degrading phosphodiesterase
Function	degradation of c-di-GMP
MW, pI	47 kDa, 5.36
Gene length, protein length	1227 bp, 409 aa
Immediate neighbours	yuzC, pncB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU31740

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (2R)-2-phospho-3-sulfolactate + H₂O = (2R)-3-sulfolactate + phosphate (according to Swiss-Prot)

• Protein family: comB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • contains an EAL domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P14203

• KEGG entry: [3]

• E.C. number: 3.1.3.71

Expression and regulation

• Operon: yuxH PubMed

• Expression browser: yuxH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Masaya Fujita, José Eduardo González-Pastor, Richard Losick
High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.
J Bacteriol: 2005, 187(4);1357-68
[PubMed:15687200] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Y Weinrauch, N Guillen, D A Dubnau
Sequence and transcription mapping of Bacillus subtilis competence genes comB and comA, one of which is related to a family of bacterial regulatory determinants.
J Bacteriol: 1989, 171(10);5362-75
[PubMed:2507523] [WorldCat.org] [DOI] (P p)