pdeH

168

c-di-GMP degrading phosphodiesterase

Locus: BSU_31740

Molecular weight: 47.76 kDa

Isoelectric point: 5.36

Protein length: 409 aa

Gene length: 1230 bp

Function: degradation of c-di-GMP

Product: c-di-GMP degrading phosphodiesterase

Essential: no

E.C.: 3.1.3.71

Synonyms: pdeH, yuxH, comB, yufA

Genomic Context

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List of homologs in different organisms, belongs to COG3434 (Galperin et al., 2021)

Gene

Coordinates: 3,258,037  3,259,266

Phenotypes of a mutant

• about three-fold increase of intracellular levels of c-di-GMP PubMed
• defective in Swarming motility PubMed

Additional information

• the pdeH gene is often inactivated during adaptation to plants PubMed

The protein

Catalyzed reaction/ biological activity

• degradation of c-di-GMP PubMed

Domains

• contains an EAL domain PubMed
• EAL domain (aa 1-209) (according to UniProt)
• HDOD domain (aa 203-392) (according to UniProt)

Structure

• P14203 (AlphaFold)

Expression and Regulation

Operons

• Genes: pdeH

  Description: PubMed

  Regulation

  • repressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • Spo0A: repression, PubMed, in spo0A regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A606 (yufA/yuxH::erm), available at the NBRP B. subtilis, Japan
• GP1590 (kan) available in Jörg Stülke's lab
• BKE31740 (pdeH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATTTATCCCCCTTAC,  downstream forward: _UP4_TATTTAGAGGCTCTGGAATG
• BKK31740 (pdeH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATTTATCCCCCTTAC,  downstream forward: _UP4_TATTTAGAGGCTCTGGAATG

References

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