Difference between revisions of "CdaA"
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* '''Mutant:''' | * '''Mutant:''' | ||
| − | ** GP997 (''[[ybbP]]''::''cat''), available in [[Stülke]] lab | + | ** GP997 (''[[ybbP]]''::''cat''), available in [[Jörg Stülke]]'s lab |
| − | ** GP985 (''[[ybbP]]''-''[[ybbR]]''::''cat''), available in [[Stülke]] lab | + | ** GP985 (''[[ybbP]]''-''[[ybbR]]''::''cat''), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| Line 131: | Line 131: | ||
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab. Respective plasmid: pGP1990. | + | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab. Respective plasmid: pGP1990. |
| − | * '''FLAG-tag construct:''' GP1331 (''ybbP''-3xFLAG ermC ''lacA''::(''ybbR''-Strep aphA3), based on [[pGP1087]]), available in | + | * '''FLAG-tag construct:''' GP1331 (''[[ybbP]]''-3xFLAG ermC ''[[lacA]]''::(''[[ybbR]]''-Strep aphA3), based on [[pGP1087]]), available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
Revision as of 14:47, 28 June 2012
- Description: maybe involved in the synthesis of c-di-AMP in vegetative cells
| Gene name | ybbP |
| Synonyms | ybbQ |
| Essential | no |
| Product | unknown |
| Function | unknown |
| Metabolic function and regulation of this protein in SubtiPathways: Murein recycling | |
| MW, pI | 30 kDa, 8.074 |
| Gene length, protein length | 819 bp, 273 aa |
| Immediate neighbours | ybbM, ybbR |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
membrane proteins, metabolism of signalling nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01750
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): YojJ
Extended information on the protein
- Kinetic information:
- Domains: contains a DAC domain potentially involved in the synthesis of c-di-AMP PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: Q45589
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
Biological materials
- Mutant:
- GP997 (ybbP::cat), available in Jörg Stülke's lab
- GP985 (ybbP-ybbR::cat), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmid: pGP1990.
- FLAG-tag construct: GP1331 (ybbP-3xFLAG ermC lacA::(ybbR-Strep aphA3), based on pGP1087), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
Additional publications: PubMed
Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep: 2011, 12(6);594-601
[PubMed:21566650]
[WorldCat.org]
[DOI]
(I p)
G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008]
[WorldCat.org]
[DOI]
(P p)
