Difference between revisions of "CdaA"

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(Biological materials)
(Biological materials)
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab. Respective plasmid pGP1990.
+
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab. Respective plasmid: pGP1990.
  
 
* '''FLAG-tag construct:''' GP1331 (''ybbP''-3xFLAG ermC ''lacA''::(''ybbR''-Strep aphA3), based on [[pGP1087]]), available in the [[Stülke]] lab
 
* '''FLAG-tag construct:''' GP1331 (''ybbP''-3xFLAG ermC ''lacA''::(''ybbR''-Strep aphA3), based on [[pGP1087]]), available in the [[Stülke]] lab

Revision as of 16:41, 25 June 2012

  • Description: maybe involved in the synthesis of c-di-AMP in vegetative cells

Gene name ybbP
Synonyms ybbQ
Essential no
Product unknown
Function unknown
Metabolic function and regulation of this protein in SubtiPathways:
Murein recycling
MW, pI 30 kDa, 8.074
Gene length, protein length 819 bp, 273 aa
Immediate neighbours ybbM, ybbR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbbP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YbbP expression.png


























Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU01750

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): YojJ

Extended information on the protein

  • Kinetic information:
  • Domains: contains a DAC domain potentially involved in the synthesis of c-di-AMP PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: the ybbP-ybbR part of the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab. Respective plasmid: pGP1990.
  • FLAG-tag construct: GP1331 (ybbP-3xFLAG ermC lacA::(ybbR-Strep aphA3), based on pGP1087), available in the Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Original publications

Additional publications: PubMed