PhrF

• Description: response regulator aspartate phosphatase (RapF) regulator, antagonizes the RapF-ComA interaction
  
  

Gene name	phrF
Synonyms	ywhI
Essential	no
Product	phosphatase (RapF) regulator
Function	control of ComA activity
Gene expression levels in SubtiExpress: phrF
Interactions involving this protein in SubtInteract: PhrF
MW, pI	4 kDa, 10.237
Gene length, protein length	117 bp, 39 aa
Immediate neighbours	rapF, ywhH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, transcription factors and their control, quorum sensing, short peptides

This gene is a member of the following regulons

ComA regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU37470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds to RapF, this results in the inability of RapF to interact with ComA PubMed

• Protein family: phr family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RapF-PhrF PubMed

• Localization:

Database entries

• Structure:

• UniProt: P71001

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rapF-phrF PubMed
  • phrF PubMed

• Expression browser: phrF PubMed

• Sigma factor:
  • phrF: SigH PubMed

• Regulation:
  • PhrF accumulates at high cell density

• Regulatory mechanism:
  • ComA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Melinda D Baker, Matthew B Neiditch
Structural basis of response regulator inhibition by a bacterial anti-activator protein.
PLoS Biol: 2011, 9(12);e1001226
[PubMed:22215984] [WorldCat.org] [DOI] (I p)

Jennifer M Auchtung, Catherine A Lee, Alan D Grossman
Modulation of the ComA-dependent quorum response in Bacillus subtilis by multiple Rap proteins and Phr peptides.
J Bacteriol: 2006, 188(14);5273-85
[PubMed:16816200] [WorldCat.org] [DOI] (P p)

Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051] [WorldCat.org] [DOI] (P p)

Cristina Bongiorni, Shu Ishikawa, Sophie Stephenson, Naotake Ogasawara, Marta Perego
Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems.
J Bacteriol: 2005, 187(13);4353-61
[PubMed:15968044] [WorldCat.org] [DOI] (P p)

R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295] [WorldCat.org] [DOI] (P p)