LipA

• Description: trigger enzyme: lipoic acid synthase and regulator of comE operon expression
  
  

Gene name	lipA
Synonyms	yutB
Essential	Yes PubMed
Product	trigger enzyme: lipoic acid synthase
Function	lipid metabolism, required for the synthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: lipA
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	33 kDa, 8.251
Gene length, protein length	894 bp, 298 aa
Immediate neighbours	yutC, lytH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, trigger enzyme

The gene

Basic information

• Locus tag: BSU32330

Phenotypes of a mutant

reduced transformation efficiency PubMed, strong inhibition of growth on minimal media PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H₂O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for comEA transcription PubMed

• Protein family: Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: O32129
• KEGG entry: [2]

• E.C. number: 2.8.1.8 3.1.1.3]

Expression and regulation

• Operon:

• Expression browser: lipA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: