ComFA

• Description: late competence protein required for DNA binding and uptake
  
  

Gene name	comFA
Synonyms
Essential	no
Product	ATP-binding protein
Function	genetic competence
Interactions involving this protein in SubtInteract: ComFA
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	52 kDa, 9.824
Gene length, protein length	1389 bp, 463 aa
Immediate neighbours	comFB, yviA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, membrane proteins, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU35470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: helicase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-20, Arg-186, and Arg-446 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ComGA-ComFA PubMed
  • ComEC-ComFA PubMed
  • CoiA-ComFA PubMed
  • SsbB-ComFA PubMed
  • DprA-ComFA PubMed
  • RecA-ComFA PubMed

• Localization:
  • cell membrane PubMed
  • localizes to cell poles in competent cells PubMed
  • localizes to cell poles PubMed

Database entries

• Structure:

• UniProt: P39145

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comFA-comFB-comFC-yvyF-flgM-yvyG-flgK-flgL PubMed

• Expression browser: comFA PubMed

• Sigma factor: comFA: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)

Original publications

Additional publications: PubMed