SirA

• Description: sporulation protein, inhibits DNA replication
  
  

Gene name	sirA
Synonyms	yneE, yoxF
Essential	no
Product	inhibitor of DNA replication
Function	control of chromosome copy number
Gene expression levels in SubtiExpress: sirA
Interactions involving this protein in SubtInteract: SirA
MW, pI	17 kDa, 7.353
Gene length, protein length	444 bp, 148 aa
Immediate neighbours	tkt, yneF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication

This gene is a member of the following regulons

Spo0A regulon

The gene

yneE was renamed to sirA.

Basic information

• Locus tag: BSU17900

Phenotypes of a mutant

"over-replication" during sporulation PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: interacts with DnaA and displaces it from the replication origin, resulting in inhibition of replication PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SirA-DnaA PubMed

• Localization:

Database entries

• Structure:

• UniProt: P45707

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sirA-yneF PubMed

• Expression browser: sirA PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Your additional remarks

sirA (sporulation inhibitor of replication) is activated by Spo0A~P and inhibits DNA replication during sporulation.

References

Reviews

Tsutomu Katayama, Shogo Ozaki, Kenji Keyamura, Kazuyuki Fujimitsu
Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.
Nat Rev Microbiol: 2010, 8(3);163-70
[PubMed:20157337] [WorldCat.org] [DOI] (I p)

Original publications

Lilah Rahn-Lee, Houra Merrikh, Alan D Grossman, Richard Losick
The sporulation protein SirA inhibits the binding of DnaA to the origin of replication by contacting a patch of clustered amino acids.
J Bacteriol: 2011, 193(6);1302-7
[PubMed:21239581] [WorldCat.org] [DOI] (I p)

Jennifer K Wagner, Kathleen A Marquis, David Z Rudner
SirA enforces diploidy by inhibiting the replication initiator DnaA during spore formation in Bacillus subtilis.
Mol Microbiol: 2009, 73(5);963-74
[PubMed:19682252] [WorldCat.org] [DOI] (I p)

Lilah Rahn-Lee, Boris Gorbatyuk, Ole Skovgaard, Richard Losick
The conserved sporulation protein YneE inhibits DNA replication in Bacillus subtilis.
J Bacteriol: 2009, 191(11);3736-9
[PubMed:19329632] [WorldCat.org] [DOI] (I p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

T Schiött, C von Wachenfeldt, L Hederstedt
Identification and characterization of the ccdA gene, required for cytochrome c synthesis in Bacillus subtilis.
J Bacteriol: 1997, 179(6);1962-73
[PubMed:9068642] [WorldCat.org] [DOI] (P p)