PanB

• Description: 3-methyl-2-oxobutanoate hydroxymethyltransferase
  
  

Gene name	panB
Synonyms
Essential	no
Product	3-methyl-2-oxobutanoate hydroxymethyltransferase
Function	biosynthesis of coenzyme A
Metabolic function and regulation of this protein in SubtiPathways: Coenzyme A
MW, pI	29 kDa, 5.282
Gene length, protein length	831 bp, 277 aa
Immediate neighbours	panC, birA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU22430

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H₂O = tetrahydrofolate + 2-dehydropantoate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3EZ4 (from Burkholderia pseudomallei, 44% identity, 64% similarity)

• UniProt: P52996

• KEGG entry: [2]

• E.C. number: 2.1.2.11

Expression and regulation

• Operon:

• Expression browser: panB PubMed

• Sigma factor:

• Regulation:
  • induced upon fatty acid biosynthesis inhibition PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Michaela Wenzel, Malay Patra, Dirk Albrecht, David Y-K Chen, K C Nicolaou, Nils Metzler-Nolte, Julia E Bandow
Proteomic signature of fatty acid biosynthesis inhibition available for in vivo mechanism-of-action studies.
Antimicrob Agents Chemother: 2011, 55(6);2590-6
[PubMed:21383089] [WorldCat.org] [DOI] (I p)