CotT

• Description: spore coat protein (inner)
  
  

Gene name	cotT
Synonyms
Essential	no
Product	spore coat protein (inner)
Function	resistance of the spore
MW, pI	12 kDa, 9.466
Gene length, protein length	321 bp, 107 aa
Immediate neighbours	ctaO, pdaC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU12090

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • inner spore coat PubMed

Database entries

• Structure:

• UniProt: P11863

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotT PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Arthur Aronson, Purdue University, West Lafayette, USA homepage

References

Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

H Takamatsu, T Kodama, A Imamura, K Asai, K Kobayashi, T Nakayama, N Ogasawara, K Watabe
The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition.
J Bacteriol: 2000, 182(7);1883-8
[PubMed:10714992] [WorldCat.org] [DOI] (P p)

N Bourne, P C FitzJames, A I Aronson
Structural and germination defects of Bacillus subtilis spores with altered contents of a spore coat protein.
J Bacteriol: 1991, 173(20);6618-25
[PubMed:1917883] [WorldCat.org] [DOI] (P p)

A I Aronson, H Y Song, N Bourne
Gene structure and precursor processing of a novel Bacillus subtilis spore coat protein.
Mol Microbiol: 1989, 3(3);437-44
[PubMed:2546006] [WorldCat.org] [DOI] (P p)