RbsD

• Description: ribose ABC transporter (membrane protein)
  
  

Gene name	rbsD
Synonyms
Essential	no
Product	ribose ABC transporter (membrane protein)
Function	ribose uptake
Interactions involving this protein in SubtInteract: RbsD
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	14 kDa, 4.987
Gene length, protein length	393 bp, 131 aa
Immediate neighbours	rbsK, rbsA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

ABC transporters, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

AbrB regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU35930

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RbsD subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RbsA(2)-(RbsC-RbsD)-RbsB PubMed

• Localization: cell membrane PubMed

Database entries

• Structure: 1OGD (complex with D-ribose)

• UniProt: P36946

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rbsR-rbsK-rbsD-rbsA-rbsC-rbsB PubMed

• Expression browser: rbsD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • expressed upon transition into the stationary phase (AbrB) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Le Thi Tam, Christine Eymann, Dirk Albrecht, Rabea Sietmann, Frieder Schauer, Michael Hecker, Haike Antelmann
Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis.
Environ Microbiol: 2006, 8(8);1408-27
[PubMed:16872404] [WorldCat.org] [DOI] (P p)

Min-Sung Kim, Joon Shin, Weontae Lee, Heung-Soo Lee, Byung-Ha Oh
Crystal structures of RbsD leading to the identification of cytoplasmic sugar-binding proteins with a novel folding architecture.
J Biol Chem: 2003, 278(30);28173-80
[PubMed:12738765] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

M A Strauch
AbrB modulates expression and catabolite repression of a Bacillus subtilis ribose transport operon.
J Bacteriol: 1995, 177(23);6727-31
[PubMed:7592460] [WorldCat.org] [DOI] (P p)

K Woodson, K M Devine
Analysis of a ribose transport operon from Bacillus subtilis.
Microbiology (Reading): 1994, 140 ( Pt 8);1829-38
[PubMed:7921236] [WorldCat.org] [DOI] (P p)

M O'Reilly, K Woodson, B C Dowds, K M Devine
The citrulline biosynthetic operon, argC-F, and a ribose transport operon, rbs, from Bacillus subtilis are negatively regulated by Spo0A.
Mol Microbiol: 1994, 11(1);87-98
[PubMed:7511775] [WorldCat.org] [DOI] (P p)