DegQ

• Description: stimulates production of degradative enzymes and of extracellular poly-gamma-glutamate PubMed, stimulates phosphorylation of DegU by DegS PubMed, gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed
  
  

Gene name	degQ
Synonyms	sacQ
Essential	no
Product	pleiotropic regulator
Function	regulation of exoenzyme synthesis
Interactions involving this protein in SubtInteract: DegQ
MW, pI	5 kDa, 6.536
Gene length, protein length	138 bp, 46 aa
Immediate neighbours	comQ, yuzC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biofilm formation, transcription factors and their control

This gene is a member of the following regulons

ComA regulon, DegU regulon

The gene

Basic information

• Locus tag: BSU31720

Phenotypes of a mutant

loss of poly-gamma-glutamate production PubMed, no synthesis of nonribosomal peptides iturin A and plipastatin PubMed1 PubMed2, reduced synthesis of degradative enzymes PubMed, increased genetic competence PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• Correction of sfp, epsC, swrAA, and degQ as well as introduction of rapP from a plasmid present in NCIB3610 results in biofilm formation in B. subtilis 168 PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • stimulates autophosphorylation of DegS PubMed

• Protein family: degQ family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DegQ-DegS PubMed

• Localization:

Database entries

• Structure:

• UniProt: Q99039

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: degQ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by high cell density (ComA) PubMed
  • subject to carbon catabolite repression PubMed
  • controlled by DegU PubMed

• Regulatory mechanism:
  • ComA: transcription activation PubMed
  • DegU: transcription activation PubMed

• Additional information: the gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed

Biological materials

• Mutant: QB4851 (cat), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Anna L McLoon, Sarah B Guttenplan, Daniel B Kearns, Roberto Kolter, Richard Losick
Tracing the domestication of a biofilm-forming bacterium.
J Bacteriol: 2011, 193(8);2027-34
[PubMed:21278284] [WorldCat.org] [DOI] (I p)

Nobuchika Takesue, Teruo Sone, Michiko Tanaka, Fusao Tomita, Kozo Asano
Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system.
J Biosci Bioeng: 2009, 107(6);623-9
[PubMed:19447339] [WorldCat.org] [DOI] (I p)

Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253] [WorldCat.org] [DOI] (P p)

Nicola R Stanley, Beth A Lazazzera
Defining the genetic differences between wild and domestic strains of Bacillus subtilis that affect poly-gamma-dl-glutamic acid production and biofilm formation.
Mol Microbiol: 2005, 57(4);1143-58
[PubMed:16091050] [WorldCat.org] [DOI] (P p)

T Msadek, F Kunst, A Klier, G Rapoport
DegS-DegU and ComP-ComA modulator-effector pairs control expression of the Bacillus subtilis pleiotropic regulatory gene degQ.
J Bacteriol: 1991, 173(7);2366-77
[PubMed:1901055] [WorldCat.org] [DOI] (P p)

H Shimotsu, D J Henner
Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.
J Bacteriol: 1986, 168(1);380-8
[PubMed:2428811] [WorldCat.org] [DOI] (P p)

M Yang, E Ferrari, E Chen, D J Henner
Identification of the pleiotropic sacQ gene of Bacillus subtilis.
J Bacteriol: 1986, 166(1);113-9
[PubMed:3007431] [WorldCat.org] [DOI] (P p)