SigG

• Description: RNA polymerase sporulation forespore-specific (late) sigma factor SigG
  
  

Gene name	sigG
Synonyms	spoIIIG
Essential	no
Product	RNA polymerase sporulation forespore-specific (late) sigma factor SigG
Function	transcription of sporulation genes (late forespore)
MW, pI	29 kDa, 5.496
Gene length, protein length	780 bp, 260 aa
Immediate neighbours	sigE, ylmA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription, sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

AbrB regulon, SigF regulon, SigG regulon, SinR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU15330

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: Gin inhibits SigG activity PubMed

• Interactions:
  • SigG-RpoB-RpoC
  • Gin-SigG PubMed, SpoIIAB-SigG PubMed

• Localization:

Database entries

• Structure:

• UniProt: P19940

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • spoIIGA-sigE-sigG PubMed
  • sigG PubMed

• Sigma factor:
  • spoIIGA: SigA PubMed
  • sigG: SigF, SigG PubMed

• Regulation:
  • spoIIGA: expressed under conditions that trigger sporulation (Spo0A) PubMed
  • sigG: expressed during sporulation (SigF, SigG) PubMed

• Regulatory mechanism:
  • repression by SinR
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Charles Moran, Emory University, NC, USA homepage

References

Reviews

Michiel J L de Hoon, Patrick Eichenberger, Dennis Vitkup
Hierarchical evolution of the bacterial sporulation network.
Curr Biol: 2010, 20(17);R735-45
[PubMed:20833318] [WorldCat.org] [DOI] (I p)

The SigG regulon

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed

Lamya Rhayat, Sandra Duperrier, Rut Carballido-López, Olivier Pellegrini, Patrick Stragier
Genetic dissection of an inhibitor of the sporulation sigma factor sigma(G).
J Mol Biol: 2009, 390(5);835-44
[PubMed:19497328] [WorldCat.org] [DOI] (I p)

Amy H Camp, Richard Losick
A feeding tube model for activation of a cell-specific transcription factor during sporulation in Bacillus subtilis.
Genes Dev: 2009, 23(8);1014-24
[PubMed:19390092] [WorldCat.org] [DOI] (I p)

Mónica Serrano, Filipe Vieira, Charles P Moran, Adriano O Henriques
Processing of a membrane protein required for cell-to-cell signaling during endospore formation in Bacillus subtilis.
J Bacteriol: 2008, 190(23);7786-96
[PubMed:18820020] [WorldCat.org] [DOI] (I p)

Amy H Camp, Richard Losick
A novel pathway of intercellular signalling in Bacillus subtilis involves a protein with similarity to a component of type III secretion channels.
Mol Microbiol: 2008, 69(2);402-17
[PubMed:18485064] [WorldCat.org] [DOI] (I p)

Takao Igarashi, Peter Setlow
Transcription of the Bacillus subtilis gerK operon, which encodes a spore germinant receptor, and comparison with that of operons encoding other germinant receptors.
J Bacteriol: 2006, 188(11);4131-6
[PubMed:16707705] [WorldCat.org] [DOI] (P p)

Louise Evans, Andrea Feucht, Jeff Errington
Genetic analysis of the Bacillus subtilis sigG promoter, which controls the sporulation-specific transcription factor sigma G.
Microbiology (Reading): 2004, 150(Pt 7);2277-2287
[PubMed:15256570] [WorldCat.org] [DOI] (P p)

Mónica Serrano, Alexandre Neves, Cláudio M Soares, Charles P Moran, Adriano O Henriques
Role of the anti-sigma factor SpoIIAB in regulation of sigmaG during Bacillus subtilis sporulation.
J Bacteriol: 2004, 186(12);4000-13
[PubMed:15175314] [WorldCat.org] [DOI] (P p)

Louise Evans, Joanna Clarkson, Michael D Yudkin, Jeff Errington, Andrea Feucht
Analysis of the interaction between the transcription factor sigmaG and the anti-sigma factor SpoIIAB of Bacillus subtilis.
J Bacteriol: 2003, 185(15);4615-9
[PubMed:12867473] [WorldCat.org] [DOI] (P p)

Mónica Serrano, Luísa Côrte, Jason Opdyke, Charles P Moran, Adriano O Henriques
Expression of spoIIIJ in the prespore is sufficient for activation of sigma G and for sporulation in Bacillus subtilis.
J Bacteriol: 2003, 185(13);3905-17
[PubMed:12813085] [WorldCat.org] [DOI] (P p)

A Cabrera-Hernandez, P Setlow
Analysis of the regulation and function of five genes encoding small, acid-soluble spore proteins of Bacillus subtilis.
Gene: 2000, 248(1-2);169-81
[PubMed:10806362] [WorldCat.org] [DOI] (P p)

I Bagyan, B Setlow, P Setlow
New small, acid-soluble proteins unique to spores of Bacillus subtilis: identification of the coding genes and regulation and function of two of these genes.
J Bacteriol: 1998, 180(24);6704-12
[PubMed:9852018] [WorldCat.org] [DOI] (P p)

C Fabret, J A Hoch
A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy.
J Bacteriol: 1998, 180(23);6375-83
[PubMed:9829949] [WorldCat.org] [DOI] (P p)

M Gomez, S M Cutting
Expression of the Bacillus subtilis spoIVB gene is under dual sigma F/sigma G control.
Microbiology (Reading): 1996, 142 ( Pt 12);3453-7
[PubMed:9004507] [WorldCat.org] [DOI] (P p)

E M Kellner, A Decatur, C P Moran
Two-stage regulation of an anti-sigma factor determines developmental fate during bacterial endospore formation.
Mol Microbiol: 1996, 21(5);913-24
[PubMed:8885263] [WorldCat.org] [DOI] (P p)

R Schmidt, A L Decatur, P N Rather, C P Moran, R Losick
Bacillus subtilis lon protease prevents inappropriate transcription of genes under the control of the sporulation transcription factor sigma G.
J Bacteriol: 1994, 176(21);6528-37
[PubMed:7961403] [WorldCat.org] [DOI] (P p)

D Foulger, J Errington
Effects of new mutations in the spoIIAB gene of Bacillus subtilis on the regulation of sigma F and sigma G activities.
J Gen Microbiol: 1993, 139(12);3197-203
[PubMed:8126438] [WorldCat.org] [DOI] (P p)

S R Partridge, J Errington
The importance of morphological events and intercellular interactions in the regulation of prespore-specific gene expression during sporulation in Bacillus subtilis.
Mol Microbiol: 1993, 8(5);945-55
[PubMed:8355618] [WorldCat.org] [DOI] (P p)

J Errington, L Appleby, R A Daniel, H Goodfellow, S R Partridge, M D Yudkin
Structure and function of the spoIIIJ gene of Bacillus subtilis: a vegetatively expressed gene that is essential for sigma G activity at an intermediate stage of sporulation.
J Gen Microbiol: 1992, 138(12);2609-18
[PubMed:1487728] [WorldCat.org] [DOI] (P p)

N Illing, J Errington
Genetic regulation of morphogenesis in Bacillus subtilis: roles of sigma E and sigma F in prespore engulfment.
J Bacteriol: 1991, 173(10);3159-69
[PubMed:1902463] [WorldCat.org] [DOI] (P p)

D X Sun, R M Cabrera-Martinez, P Setlow
Control of transcription of the Bacillus subtilis spoIIIG gene, which codes for the forespore-specific transcription factor sigma G.
J Bacteriol: 1991, 173(9);2977-84
[PubMed:1902213] [WorldCat.org] [DOI] (P p)

P Fajardo-Cavazos, F Tovar-Rojo, P Setlow
Effect of promoter mutations and upstream deletions on the expression of genes coding for small, acid-soluble spore proteins of Bacillus subtilis.
J Bacteriol: 1991, 173(6);2011-6
[PubMed:1900507] [WorldCat.org] [DOI] (P p)

Y Nakatani, W L Nicholson, K D Neitzke, P Setlow, E Freese
Sigma-G RNA polymerase controls forespore-specific expression of the glucose dehydrogenase operon in Bacillus subtilis.
Nucleic Acids Res: 1989, 17(3);999-1017
[PubMed:2493633] [WorldCat.org] [DOI] (P p)

D X Sun, P Stragier, P Setlow
Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis.
Genes Dev: 1989, 3(2);141-9
[PubMed:2497051] [WorldCat.org] [DOI] (P p)

R M Jonas, E A Weaver, T J Kenney, C P Moran, W G Haldenwang
The Bacillus subtilis spoIIG operon encodes both sigma E and a gene necessary for sigma E activation.
J Bacteriol: 1988, 170(2);507-11
[PubMed:2448286] [WorldCat.org] [DOI] (P p)