FadN

• Description: 3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
  
  

Gene name	fadN
Synonyms	yusL
Essential	no
Product	3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
Function	fatty acid degradation
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	89 kDa, 6.53
Gene length, protein length	2445 bp, 815 aa
Immediate neighbours	fadA, fadM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

FadR regulon, SdpR regulon

The gene

Basic information

• Locus tag: BSU32840

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-3-hydroxyacyl-CoA + NAD⁺ = 3-oxoacyl-CoA + NADH (according to Swiss-Prot)

• Protein family: 3-hydroxyacyl-CoA dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O32178

• KEGG entry: [3]

• E.C. number: 1.1.1.35

Expression and regulation

• Operon:
  • fadM-fadN-fadA-fadE PubMed
  • fadN-fadA-fadE PubMed

• Sigma factor:
  • fadN: SigA PubMed

• Regulation:
  • fadN: induced by long-chain fatty acids (FadR) PubMed
  • fadN: subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed
  • SdpR: transcription activation PubMed
  • CcpA-HPr(Ser-P): transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed