Difference between revisions of "YvcJ"

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* '''Description:''' nucleotide-binding protein <br/><br/>
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* '''Description:''' GTPase, nucleotide-binding protein <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || nucleotide-binding P-loop-containing protein
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|style="background:#ABCDEF;" align="center"| '''Product''' || GTPase
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || required for the expression of late competence genes
 
|style="background:#ABCDEF;" align="center"|'''Function''' || required for the expression of late competence genes
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
  
reduced competence  [http://www.ncbi.nlm.nih.gov/sites/entrez/19074378 PubMed]
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* reduced [[genetic competence]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19074378 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Localization:'''
 
* '''Localization:'''
 +
** can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium {{PubMed|21709426}}
  
 
=== Database entries ===
 
=== Database entries ===
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=References=
 
=References=
 
==Original publications==
 
==Original publications==
<pubmed>9237995 16272399 19074378, </pubmed>
+
<pubmed>9237995 16272399 19074378, 21709426 </pubmed>
 
==Publications on the ''E. coli'' homolog==
 
==Publications on the ''E. coli'' homolog==
 
<pubmed>17824929 , </pubmed>
 
<pubmed>17824929 , </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:25, 29 June 2011

  • Description: GTPase, nucleotide-binding protein

Gene name yvcJ
Synonyms
Essential no
Product GTPase
Function required for the expression of late competence genes
MW, pI 33 kDa, 6.702
Gene length, protein length 885 bp, 295 aa
Immediate neighbours yvcK, yvcI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvcJ context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

genetic competence

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU34770

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds and hydrolyzes nucleotides, required for the expression of late competence genes (comK, comS) PubMed
  • Protein family: UPF0042 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:
    • can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector: pGP735 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • Antibody:

Labs working on this gene/protein

Anne Galinier, University of Marseille, France

Your additional remarks

References

Original publications

Publications on the E. coli homolog

Falk Kalamorz, Birte Reichenbach, Walter März, Bodo Rak, Boris Görke
Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli.
Mol Microbiol: 2007, 65(6);1518-33
[PubMed:17824929] [WorldCat.org] [DOI] (P p)