Difference between revisions of "NucA"
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| + | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[genetic competence]]}}, | ||
| + | {{SubtiWiki category|[[membrane proteins]]}} | ||
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| + | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[ComK regulon]]}} | ||
=The gene= | =The gene= | ||
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=The protein= | =The protein= | ||
Revision as of 16:37, 8 December 2010
- Description: membrane-associated nuclease, catalyzes DNA cleavage during transformation
| Gene name | nucA |
| Synonyms | comI |
| Essential | no |
| Product | membrane-associated nuclease |
| Function | genetic transformation, DNA uptake |
| MW, pI | 16 kDa, 4.677 |
| Gene length, protein length | 441 bp, 147 aa |
| Immediate neighbours | nin, tlpC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
genetic competence, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU03430
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot), integral membrane protein PubMed
Database entries
- Structure:
- UniProt: P12667
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663]
[WorldCat.org]
[DOI]
(P p)
R Provvedi, I Chen, D Dubnau
NucA is required for DNA cleavage during transformation of Bacillus subtilis.
Mol Microbiol: 2001, 40(3);634-44
[PubMed:11359569]
[WorldCat.org]
[DOI]
(P p)
D van Sinderen, R Kiewiet, G Venema
Differential expression of two closely related deoxyribonuclease genes, nucA and nucB, in Bacillus subtilis.
Mol Microbiol: 1995, 15(2);213-23
[PubMed:7746143]
[WorldCat.org]
[DOI]
(P p)
