Difference between revisions of "MalR"

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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[utilization of specific carbon sources]]}},
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{{SubtiWiki category|[[transcription factors and their control]]}},
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{{SubtiWiki category|[[phosphoproteins]]}}
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= This gene is a member of the following [[regulons]] =
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=The gene=
 
=The gene=
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[utilization of specific carbon sources]]}},
 
{{SubtiWiki category|[[transcription factors and their control]]}},
 
{{SubtiWiki category|[[phosphoproteins]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 22:54, 8 December 2010

  • Description: two-component response regulator, regulation of malate uptake

Gene name malR
Synonyms yufM
Essential no
Product two-component response regulator
Function regulation of malate uptake
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 26 kDa, 5.976
Gene length, protein length 705 bp, 235 aa
Immediate neighbours malK, yufN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YufM context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU31530

Phenotypes of a mutant

unable to use malate as the single carbon source PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Genes/ operons controlled by MalR

maeA, maeN, yflS

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated by MalK on an Asp residue
  • Cofactor(s):
  • Effectors of protein activity: phosphorylation likely affects DNA-binding activity
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References

Thierry Doan, Pascale Servant, Shigeo Tojo, Hirotake Yamaguchi, Guillaume Lerondel, Ken-Ichi Yoshida, Yasutaro Fujita, Stéphane Aymerich
The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate.
Microbiology (Reading): 2003, 149(Pt 9);2331-2343
[PubMed:12949160] [WorldCat.org] [DOI] (P p)

Kousei Tanaka, Kazuo Kobayashi, Naotake Ogasawara
The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium.
Microbiology (Reading): 2003, 149(Pt 9);2317-2329
[PubMed:12949159] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Gregory Koningstein, Luísa Rodrigues, Maria de Sales Ramon, Helmut Hilbert, Andreas Düsterhöft, Thomas M Pohl, Thomas Weitzenegger
Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).
Microbiology (Reading): 1997, 143 ( Pt 8);2769-2774
[PubMed:9274030] [WorldCat.org] [DOI] (P p)