SpoIIE

• Description: protein serine phosphatase, septum-associated PP2C, dephosphorylation of SpoIIAA
  
  

Gene name	spoIIE
Synonyms	spoIIH, spoIIK
Essential	no
Product	protein serine phosphatase, septum-associated PP2C
Function	control of SigF activity required for normal formation of the asymmetric septum
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	91 kDa, 6.137
Gene length, protein length	2481 bp, 827 aa
Immediate neighbours	trnSL-Glu1, yabS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00640

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

protein modification, sigma factors and their control, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A phosphoprotein + H₂O = a protein + phosphate (according to Swiss-Prot) dephosphorylation of SpoIIAA

• Protein family: PP2C phosphatase

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SpoIIE-FtsZ, SpoIIE-SpoIIAB, SpoIIE-SpoIIE, SpoIIQ-SpoIIE

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P37475

• KEGG entry: [3]

• E.C. number: 3.1.3.16

Expression and regulation

• Operon: spoIIE (DBTBS)

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage