RibE

• Description: riboflavin synthase (alpha subunit)
  
  

Gene name	ribE
Synonyms	ribB
Essential	no
Product	riboflavin synthase (alpha subunit)
Function	riboflavin biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Riboflavin / FAD
MW, pI	23 kDa, 5.845
Gene length, protein length	645 bp, 215 aa
Immediate neighbours	ribA, ribD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU23270

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis of cofactors

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: (RibE)3-(RibH)60, the lumazine synthase/riboflavin synthase complex PubMed

• Localization:

Database entries

• Structure: 1I8D (from E. coli, 36% identity, 59% similarity) PubMed, , 1RVV (the RibE)3-(RibH)60 lumazine synthase/riboflavin synthase complex) PubMed

• UniProt: P16440

• KEGG entry: [3]

• E.C. number: 2.5.1.9

Expression and regulation

• Operon: ypuE-ribD-ribE-ribA-ribH-ribT PubMed

• Sigma factor: SigA PubMed

• Regulation: expressed in the absence of FMN (FMN-box) PubMed

• Regulatory mechanism: FMN-box: riboswitch, mediates termination/ antitermination control of the operon, in the absence of FMN: antitermination, in the presence of FMN: termination PubMed

• Additional information:

Biological materials

• Mutant: GP203 (erm), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: