PucR

• Description: transcriptional regulator of puc genes
  
  

Gene name	pucR
Synonyms	yunI
Essential	no
Product	transcriptional regulator of puc genes
Function	regulation of purine utilization
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	60 kDa, 6.188
Gene length, protein length	1593 bp, 531 aa
Immediate neighbours	pucH, pucJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU32420

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: cdaR family (according to Swiss-Prot)

• Paralogous protein(s):

Genes/ operons controlled by PucR PubMed

• Activation by PucR: guaD, pucF-pucG, pucH, pucI, pucJ-pucK-pucL-pucM

• Repression by PucR: pucA-pucB-pucC-pucD-pucE, pucR

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O32138

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pucR-pucJ-pucK-pucL-pucM PubMed

• Sigma factor:

• Regulation:
  • repressed by purine nucleotides (effector: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • PucR: transcription auto-repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: