Difference between revisions of "DegU"

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(References)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''''' [[degS]]-[[degU]]''
+
* '''Operon:''''' [[degS]]-[[degU]]'' {{PubMed|1688843}}
  
* '''[[Sigma factor]]:''' [[SigA]]
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1688843}}
  
* '''Regulation:''' induced in the presence of ammonium ([[TnrA]])
+
* '''Regulation:'''  
** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18502860 PubMed]
+
** induced in the presence of ammonium ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18502860 PubMed]
  
* '''Regulatory mechanism:''' [[TnrA]]: repression, [[DegU]]: positive autoregulation
+
* '''Regulatory mechanism:'''  
 +
** [[DegU]]: positive autoregulation
 
** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/18502860 PubMed]
 
** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/18502860 PubMed]
  
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<pubmed>20030732 </pubmed>
 
<pubmed>20030732 </pubmed>
 
==Original Publications==
 
==Original Publications==
<pubmed>17850253,12471443,8878039,14563871,1901568,1321152,1459944,18194340,10908654,18502860,10094672,19389763,18414485,19420703,2428811,7746142,18502860, 19389763, 19416356, 19118340 19734658 </pubmed>
+
<pubmed>17850253,12471443,8878039, 14563871,1901568,1321152,1459944, 18194340,10908654,18502860,10094672,19389763, 18414485,19420703,2428811, 7746142, 18502860, 19389763, 19416356, 19118340 19734658 1688843 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 21:51, 30 December 2009

  • Description: two-component response regulator, regulation of degradative enzyme and genetic competence, involved in activation of capsule biosynthetic operon expression (together with SwrAA), non-phosphorylated DegU is required for swarming motility PubMed

Gene name degU
Synonyms sacU, iep
Essential no
Product two-component response regulator
Function regulation of degradative enzyme and genetic competence

of genetic competence

MW, pI 25 kDa, 5.602
Gene length, protein length 687 bp, 229 aa
Immediate neighbours yviA, degS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DegU context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU35490

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription activator of degradative enzyme genes (sacB and degQ) and of comK expression
  • Protein family:
  • Paralogous protein(s):

Genes controlled by DegU

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated by DegS on Asp-56 PubMed, this modulates DNA-binding activity
  • Cofactor(s):
  • Effectors of protein activity: DNA-binding activity of DegU is inhibited by RapG PubMed
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of ammonium (TnrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Daniel López, Roberto Kolter
Extracellular signals that define distinct and coexisting cell fates in Bacillus subtilis.
FEMS Microbiol Rev: 2010, 34(2);134-49
[PubMed:20030732] [WorldCat.org] [DOI] (I p)

Original Publications