Difference between revisions of "BioI"
Line 70: | Line 70: | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
− | * '''Interactions:''' [[Fer]]-[[BioI]] {{PubMed|12538057}}, [[YkuP]]-[[BioI]] {{PubMed|15449930}}, [[AcpA]]-[[BioI]] | + | * '''Interactions:''' [[Fer]]-[[BioI]] {{PubMed|12538057}}, [[YkuP]]-[[BioI]] {{PubMed|15449930}}, [[AcpA]]-[[BioI]] {{PubMed|18838690}} |
* '''Localization:''' | * '''Localization:''' | ||
Line 76: | Line 76: | ||
=== Database entries === | === Database entries === | ||
− | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3EJE 3EJE] | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3EJE 3EJE] (complex with [[AcpA]]) {{PubMed|18838690}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/P53554 P53554] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P53554 P53554] | ||
Line 119: | Line 119: | ||
=References= | =References= | ||
− | <pubmed>,8763940,8892842,15449931, 15449930 12538057</pubmed> | + | <pubmed> 18838690 ,8763940,8892842,15449931, 15449930 12538057</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 17:42, 6 January 2010
- Description: cytochrome P450 enzyme
Gene name | bioI |
Synonyms | |
Essential | no |
Product | cytochrome P450 enzyme |
Function | biosynthesis of biotin |
MW, pI | 44 kDa, 6.2 |
Gene length, protein length | 1185 bp, 395 aa |
Immediate neighbours | ytbQ, bioB |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU30190
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: cytochrome P450 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- UniProt: P53554
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Max J Cryle, Ilme Schlichting
Structural insights from a P450 Carrier Protein complex reveal how specificity is achieved in the P450(BioI) ACP complex.
Proc Natl Acad Sci U S A: 2008, 105(41);15696-701
[PubMed:18838690]
[WorldCat.org]
[DOI]
(I p)
Rachel J Lawson, David Leys, Michael J Sutcliffe, Carol A Kemp, Myles R Cheesman, Susan J Smith, John Clarkson, W Ewen Smith, Ihtshamul Haq, John B Perkins, Andrew W Munro
Thermodynamic and biophysical characterization of cytochrome P450 BioI from Bacillus subtilis.
Biochemistry: 2004, 43(39);12410-26
[PubMed:15449931]
[WorldCat.org]
[DOI]
(P p)
Rachel J Lawson, Claes von Wachenfeldt, Ihtshamul Haq, John Perkins, Andrew W Munro
Expression and characterization of the two flavodoxin proteins of Bacillus subtilis, YkuN and YkuP: biophysical properties and interactions with cytochrome P450 BioI.
Biochemistry: 2004, 43(39);12390-409
[PubMed:15449930]
[WorldCat.org]
[DOI]
(P p)
Amanda J Green, Andrew W Munro, Myles R Cheesman, Graeme A Reid, Claes von Wachenfeldt, Stephen K Chapman
Expression, purification and characterisation of a Bacillus subtilis ferredoxin: a potential electron transfer donor to cytochrome P450 BioI.
J Inorg Biochem: 2003, 93(1-2);92-9
[PubMed:12538057]
[WorldCat.org]
[DOI]
(P p)
J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842]
[WorldCat.org]
[DOI]
(P p)
S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940]
[WorldCat.org]
[DOI]
(P p)