Difference between revisions of "Obg"

From SubtiWiki
Jump to: navigation, search
Line 12: Line 12:
 
|style="background:#ABCDEF;" align="center"| '''Product''' || GTP-binding protein  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || GTP-binding protein  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || may be required to stimulate activity of the [[phosphorelay]] that activates [[Spo0A]]
+
|style="background:#ABCDEF;" align="center"|'''Function''' || may be required to stimulate activity <br/>
 +
of the [[phosphorelay]] that activates [[Spo0A]]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 4.859   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 4.859   
Line 94: Line 95:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[spo0B]]-[[obg]]''
+
* '''Operon:''' ''[[spo0B]]-[[obg]]'' {{PubMed|2537815}}
  
* '''Sigma factor:''' [[SigA]]
+
* '''Sigma factor:''' [[SigA]] {{PubMed|2537815}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 129: Line 130:
 
=References=
 
=References=
  
<pubmed>15325267,18689482,7961487,7961486,15583165,2537815,10781545,10419966,12427945,12429099 </pubmed>
+
<pubmed>15325267,18689482,7961487,7961486,15583165,2537815,10781545,10419966, 12427945,12429099 2537815 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 18:52, 13 December 2009

  • Description: GTP-binding protein involved in initiation of sporulation

Gene name obg
Synonyms
Essential Yes
Product GTP-binding protein
Function may be required to stimulate activity

of the phosphorelay that activates Spo0A

MW, pI 47 kDa, 4.859
Gene length, protein length 1284 bp, 428 aa
Immediate neighbours pheB, spo0B
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Obg context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU27920

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: GTP1/OBG family (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:

Obg possesses 3 domains: A N-terminal glycine-rich domain (1–158), a GTP binding domain (159–342) and a C-terminal TGS domain (343–428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.

  • Modification:
  • Cofactor(s):

GDP, GTP and probably ppGpp

  • Effectors of protein activity:
  • Interactions:

Interaction with Rsb proteins.

  • Localization:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in B. subtilis.

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP835, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP843, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References