Difference between revisions of "AraR"

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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor ([[GntR family]], [[LacI family]]))
+
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor ([[GntR family]], [[LacI family]])
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of arabinose utilization  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of arabinose utilization  
(araABDLMNPQ-abfA) and of the araE/araR genes
+
(''[[araA|araABDLMNPQ-abfA]]'') and of the ''[[araE]]''/''[[araR]]'' genes
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 6.341   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 6.341   
Line 60: Line 60:
  
 
=== Genes/ operons controlled by AraR ===
 
=== Genes/ operons controlled by AraR ===
 
 
* '''Activated by AraR:'''  
 
* '''Activated by AraR:'''  
* ''' Repressed by AraR:''' ''[[araA]]-[[araB]]-[[araD]]-[[araL]]-[[araM]]-[[araN]]-[[araP]]-[[araQ]]-[[abfA]]''
+
* '''Repressed by AraR:''' ''[[araA]]-[[araB]]-[[araD]]-[[araL]]-[[araM]]-[[araN]]-[[araP]]-[[araQ]]-[[abfA]]'', ''[[araE]]'', ''[[araR]]''
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 95: Line 94:
  
 
=Expression and regulation=
 
=Expression and regulation=
 +
* '''Operon:''' ''[[araR]]'' {{PubMed|9045819}}
  
* '''Operon:'''
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9401028}}
 
 
* '''[[Sigma factor]]:'''  
 
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** repressed in the absence of arabinose ([[AraR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
+
** induced by arabinose ([[AraR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  

Revision as of 11:05, 29 December 2009

  • Description: repressor of the arabinose utilization genes

Gene name araR
Synonyms araC, yvbS
Essential no
Product transcriptional repressor (GntR family, LacI family)
Function regulation of arabinose utilization

(araABDLMNPQ-abfA) and of the araE/araR genes

MW, pI 43 kDa, 6.341
Gene length, protein length 1152 bp, 384 aa
Immediate neighbours araE, yvbT
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AraR context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU33970

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Genes/ operons controlled by AraR

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Irina Saraiva Franco, Luís Jaime Mota, Cláudio Manuel Soares, Isabel de Sá-Nogueira
Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.
Nucleic Acids Res: 2007, 35(14);4755-66
[PubMed:17617643] [WorldCat.org] [DOI] (I p)

Irina Saraiva Franco, Luís Jaime Mota, Cláudio Manuel Soares, Isabel de Sá-Nogueira
Functional domains of the Bacillus subtilis transcription factor AraR and identification of amino acids important for nucleoprotein complex assembly and effector binding.
J Bacteriol: 2006, 188(8);3024-36
[PubMed:16585763] [WorldCat.org] [DOI] (P p)

Maria Paiva Raposo, José Manuel Inácio, Luís Jaime Mota, Isabel de Sá-Nogueira
Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis.
J Bacteriol: 2004, 186(5);1287-96
[PubMed:14973026] [WorldCat.org] [DOI] (P p)

L J Mota, L M Sarmento, I de Sá-Nogueira
Control of the arabinose regulon in Bacillus subtilis by AraR in vivo: crucial roles of operators, cooperativity, and DNA looping.
J Bacteriol: 2001, 183(14);4190-201
[PubMed:11418559] [WorldCat.org] [DOI] (P p)

L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, L J Mota
Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene.
J Bacteriol: 1997, 179(5);1598-608
[PubMed:9045819] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, H Paveia, H de Lencastre
Isolation of constitutive mutants for L-arabinose utilization in Bacillus subtilis.
J Bacteriol: 1988, 170(6);2855-7
[PubMed:3131313] [WorldCat.org] [DOI] (P p)