Difference between revisions of "YqiQ"
| Line 78: | Line 78: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1MUM 1MUM] (enzyme of ''E. coli'') | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1MUM 1MUM] (enzyme of ''E. coli''), [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3KZ2 3KZ2] (the enzyme from ''B. anthracis'') |
* '''UniProt:''' [http://www.uniprot.org/uniprot/P54528 P54528] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P54528 P54528] | ||
| Line 90: | Line 90: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' [[mmgA]]-[[mmgB]]-[[mmgC]]-[[mmgD]]-[[mmgE]]-[[yqiQ]] {{PubMed|8759838}} | + | * '''Operon:''' ''[[mmgA]]-[[mmgB]]-[[mmgC]]-[[mmgD]]-[[mmgE]]-[[yqiQ]]'' {{PubMed|8759838}} |
* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15699190,8759838}} | * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15699190,8759838}} | ||
Revision as of 18:48, 6 January 2010
- Description: methylisocitrate lyase
| Gene name | yqiQ |
| Synonyms | prpB |
| Essential | no |
| Product | methylisocitrate lyase |
| Function | mother cell metabolism |
| Metabolic function and regulation of this protein in SubtiPathways: Coenzyme A, Central C-metabolism | |
| MW, pI | 32 kDa, 5.279 |
| Gene length, protein length | 903 bp, 301 aa |
| Immediate neighbours | yqzF, mmgE |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU24120
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate (according to Swiss-Prot)
- Protein family: Methylisocitrate lyase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- UniProt: P54528
- KEGG entry: [3]
- E.C. number: 4.1.3.30
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190]
[WorldCat.org]
[DOI]
(P p)
Clemens Grimm, Andreas Evers, Matthias Brock, Claudia Maerker, Gerhard Klebe, Wolfgang Buckel, Klaus Reuter
Crystal structure of 2-methylisocitrate lyase (PrpB) from Escherichia coli and modelling of its ligand bound active centre.
J Mol Biol: 2003, 328(3);609-21
[PubMed:12706720]
[WorldCat.org]
[DOI]
(P p)
E M Bryan, B W Beall, C P Moran
A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis.
J Bacteriol: 1996, 178(16);4778-86
[PubMed:8759838]
[WorldCat.org]
[DOI]
(P p)
