Difference between revisions of "MtlR"
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| − | * '''Description:''' transcriptional activator of the ''[[mtlA]]-[[mtlD]]'' operon <br/><br/> | + | * '''Description:''' transcriptional activator of the ''[[mtlA]]-[[mtlF]]-[[mtlD]]'' operon <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 64: | Line 64: | ||
* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''Domains:''' N-terminal DNA-binding domains, two [[PTS]]-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domain |
| − | * '''Modification:''' | + | * '''Modification:''' |
| + | ** phosphorylated on His-342 and/or His-399 by [[PtsH]] {{PubMed|20444094}} | ||
| + | ** phosphorylation on His-599 by [[MtlA]] {{PubMed|20444094}} | ||
| + | ** phosphorylation on Cys-419 by [[MtlF]] {{PubMed|20444094}} | ||
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| + | ** activity is stimulated by [[PtsH]]-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) {{PubMed|20444094}} | ||
| + | ** activity is inhibited by [[MtlF]]-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) {{PubMed|20444094}} | ||
| − | * '''Interactions:''' [[PtsH]]-[[MtlR]], [[MtlA]]-[[MtlR]] | + | * '''Interactions:''' [[PtsH]]-[[MtlR]] {{PubMed|20444094}}, [[MtlA]]-[[MtlR]] {{PubMed|20444094}}, [[MtlF]]-[[MtlR]] {{PubMed|20444094}} |
* '''Localization:''' | * '''Localization:''' | ||
| Line 120: | Line 125: | ||
=References= | =References= | ||
| − | <pubmed>12897001,,10627040, </pubmed> | + | <pubmed>12897001, 20444094,10627040, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:08, 11 May 2010
| Gene name | mtlR |
| Synonyms | ydaA |
| Essential | no |
| Product | transcriptional activator, PRD-type |
| Function | regulation of mannitol utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism | |
| MW, pI | 78 kDa, 5.313 |
| Gene length, protein length | 2082 bp, 694 aa |
| Immediate neighbours | ycsN, ydaB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU04160
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: N-terminal DNA-binding domains, two PTS-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domain
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- activity is stimulated by PtsH-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) PubMed
- activity is inhibited by MtlF-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) PubMed
- Localization:
Database entries
- Structure:
- UniProt: P96574
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: mtlR PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Philippe Joyet, Meriem Derkaoui, Sandrine Poncet, Josef Deutscher
Control of Bacillus subtilis mtl operon expression by complex phosphorylation-dependent regulation of the transcriptional activator MtlR.
Mol Microbiol: 2010, 76(5);1279-94
[PubMed:20444094]
[WorldCat.org]
[DOI]
(I p)
Shouji Watanabe, Miyuki Hamano, Hiroshi Kakeshita, Keigo Bunai, Shigeo Tojo, Hirotake Yamaguchi, Yasutaro Fujita, Sui-Lam Wong, Kunio Yamane
Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and gut operons.
J Bacteriol: 2003, 185(16);4816-24
[PubMed:12897001]
[WorldCat.org]
[DOI]
(P p)
Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040]
[WorldCat.org]
[DOI]
(P p)
