Difference between revisions of "BioD"
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || dethiobiotin synthase | + | |style="background:#ABCDEF;" align="center"| '''Product''' || dethiobiotin synthase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of biotin | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of biotin | ||
| Line 87: | Line 87: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | + | * '''Operon:''' ''[[bioW]]-[[bioA]]-[[bioF]]-[[bioD]]-[[bioB]]-[[bioI]]-[[ytbQ]]'' {{PubMed|8763940,8892842}} | |
| − | * '''Operon:''' | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** repressed in the presence of biotin ([[BirA]]) {{PubMed|8763940,8892842}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[BirA]]: transcription repression {{PubMed|8763940,8892842}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 19:37, 19 December 2009
- Description: dethiobiotin synthase
| Gene name | bioD |
| Synonyms | |
| Essential | no |
| Product | dethiobiotin synthase |
| Function | biosynthesis of biotin |
| MW, pI | 25 kDa, 6.209 |
| Gene length, protein length | 693 bp, 231 aa |
| Immediate neighbours | bioB, bioF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU30210
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin (according to Swiss-Prot)
- Protein family: dethiobiotin synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P53558
- KEGG entry: [3]
- E.C. number: 6.3.3.3
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842]
[WorldCat.org]
[DOI]
(P p)
S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940]
[WorldCat.org]
[DOI]
(P p)
