Difference between revisions of "PupG"
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|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''pupG'' |
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''pnp, yqkO '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''punA, pnp, yqkO '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[drm]]-[[pupG]]'' {{PubMed|10537218}} |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** induced in the presence of nucleosides (deoxyribose 5-phosphate and ribose 5-phosphate act as molecular inducers) {{PubMed|10537218}} | ||
| + | ** subject to repression by glucose {{PubMed|10537218}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
Revision as of 09:13, 13 July 2009
- Description: purine nucleoside phosphorylase
| Gene name | pupG |
| Synonyms | punA, pnp, yqkO |
| Essential | no |
| Product | purine nucleoside phosphorylase |
| Function | purine salvage and interconversion |
| Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleoside catabolism, Nucleotides (regulation) | |
| MW, pI | 28 kDa, 4.86 |
| Gene length, protein length | 813 bp, 271 aa |
| Immediate neighbours | dacF, drm |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU23490
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate (according to Swiss-Prot) RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate (according to Swiss-Prot)
- Protein family: PNP/MTAP phosphorylase family (according to Swiss-Prot) polyribonucleotide nucleotidyltransferase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylation on Ser-28 PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: secreted (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: P46354
- KEGG entry: [3]
- E.C. number: 2.4.2.1 2.7.7.8]
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
