FlgN

• Description: flagellar filament assembly protein
  
  

Gene name	yvyG
Synonyms	yviC
Essential	no
Product	flagellar filament assembly protein
Function	flagellar filament assembly
Gene expression levels in SubtiExpress: flgN
Interactions involving this protein in SubtInteract: FlgN
MW, pI	18 kDa, 8.81
Gene length, protein length	480 bp, 160 aa
Immediate neighbours	flgK, flgM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, phosphoproteins

This gene is a member of the following regulons

ComK regulon, DegU regulon, SigD regulon

The gene

Basic information

• Locus tag: BSU35420

Phenotypes of a mutant

• non-motile cells PubMed

Database entries

• BsubCyc: BSU35420

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • essential for flagellar filament assembly, export of hook-filament junction proteins FlgK and FlgL PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-60 PubMed, phosphorylation does not affect the properties of the protein PubMed
  • phosphorylation on Tyr-49 PubMed, phosphorylation does not affect the properties of the protein PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU35420

• Structure:

• UniProt: P39808

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • comFA-comFB-comFC-yvyF-flgM-flgN-flgK-flgL PubMed
  • yvyF-flgM-flgN-flgK-flgL PubMed
  • flgM-flgN-flgK-flgL PubMed

• Expression browser: flgN PubMed

• Sigma factor:
  • comFA: SigA PubMed
  • yvyF: SigD PubMed
  • flgM: SigD PubMed

• Regulation:

• Regulatory mechanism:
  • ComK: transcription activation PubMed
  • flgM: DegU-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lei Shi, Boyang Ji, Lorena Kolar-Znika, Ana Boskovic, Fanny Jadeau, Christophe Combet, Christophe Grangeasse, Damjan Franjevic, Emmanuel Talla, Ivan Mijakovic
Evolution of bacterial protein-tyrosine kinases and their relaxed specificity toward substrates.
Genome Biol Evol: 2014, 6(4);800-17
[PubMed:24728941] [WorldCat.org] [DOI] (I p)

Lynne S Cairns, Victoria L Marlow, Taryn B Kiley, Christopher Birchall, Adam Ostrowski, Phillip D Aldridge, Nicola R Stanley-Wall
FlgN is required for flagellum-based motility by Bacillus subtilis.
J Bacteriol: 2014, 196(12);2216-26
[PubMed:24706744] [WorldCat.org] [DOI] (I p)

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Yi-Huang Hsueh, Loralyn M Cozy, Lok-To Sham, Rebecca A Calvo, Alina D Gutu, Malcolm E Winkler, Daniel B Kearns
DegU-phosphate activates expression of the anti-sigma factor FlgM in Bacillus subtilis.
Mol Microbiol: 2011, 81(4);1092-108
[PubMed:21736639] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Mark J Pallen, Charles W Penn, Roy R Chaudhuri
Bacterial flagellar diversity in the post-genomic era.
Trends Microbiol: 2005, 13(4);143-9
[PubMed:15817382] [WorldCat.org] [DOI] (P p)

D B Mirel, P Lauer, M J Chamberlin
Identification of flagellar synthesis regulatory and structural genes in a sigma D-dependent operon of Bacillus subtilis.
J Bacteriol: 1994, 176(15);4492-500
[PubMed:8045879] [WorldCat.org] [DOI] (P p)

J A Londoño-Vallejo, D Dubnau
comF, a Bacillus subtilis late competence locus, encodes a protein similar to ATP-dependent RNA/DNA helicases.
Mol Microbiol: 1993, 9(1);119-31
[PubMed:8412657] [WorldCat.org] [DOI] (P p)