Difference between revisions of "PucA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || purine utilization | |style="background:#ABCDEF;" align="center"|'''Function''' || purine utilization | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_catabolism.html Purine catabolism], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]''' | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_salvage_pathways.html Purine salvage], [http://subtiwiki.uni-goettingen.de/pathways/purines_catabolism.html Purine catabolism], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]''' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 6.359 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 6.359 | ||
Revision as of 13:48, 18 June 2009
- Description: xanthine dehydrogenase
| Gene name | pucA |
| Synonyms | yurF |
| Essential | no |
| Product | xanthine dehydrogenase |
| Function | purine utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Purine catabolism, Nucleotides (regulation) | |
| MW, pI | 36 kDa, 6.359 |
| Gene length, protein length | 990 bp, 330 aa |
| Immediate neighbours | pucB, pucG |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU32510
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Xanthine + NAD+ + H2O = urate + NADH (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: O32147
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- [PucR]]: transcription repression PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039]
[WorldCat.org]
[DOI]
(P p)
A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136]
[WorldCat.org]
[DOI]
(P p)
