Difference between revisions of "AraE"

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* '''Regulation:''' repressed by glucose ([[CcpA]]),  induced by arabinose [http://www.ncbi.nlm.nih.gov/sites/entrez/9620981 PubMed]
 
* '''Regulation:''' repressed by glucose ([[CcpA]]),  induced by arabinose [http://www.ncbi.nlm.nih.gov/sites/entrez/9620981 PubMed]
 +
** repressed in the absence of arabinose ([[AraR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
  
 
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression  
 
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression  
 +
** [[AraR]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 11:46, 11 June 2009

  • Description: L-arabinose permease

Gene name araE
Synonyms yvbR
Essential no
Product L-arabinose permease
Function uptake of arabinose, galactose and xylose
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 50 kDa, 9.259
Gene length, protein length 1392 bp, 464 aa
Immediate neighbours cggR, araR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AraE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU33960

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transports arabinose, and also xylose and galactose PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: repressed by glucose (CcpA), induced by arabinose PubMed
    • repressed in the absence of arabinose (AraR) PubMed
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

O Krispin, R Allmansberger
The Bacillus subtilis AraE protein displays a broad substrate specificity for several different sugars.
J Bacteriol: 1998, 180(12);3250-2
[PubMed:9620981] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, S S Ramos
Cloning, functional analysis, and transcriptional regulation of the Bacillus subtilis araE gene involved in L-arabinose utilization.
J Bacteriol: 1997, 179(24);7705-11
[PubMed:9401028] [WorldCat.org] [DOI] (P p)

  1. Krispin, O., and R. Allmansberger. (1998) The Bacillus subtilis AraE protein displays a broad substrate specificity for several different sugars. J. Bacteriol. 180: 3250-3252. PubMed
  2. Sá-Nogueira I, Ramos SS. (1997) Cloning, functional analysis, and transcriptional regulation of the Bacillus subtilis araE gene involved in L-arabinose utilization. J Bacteriol. Dec;179(24): 7705-11. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed