Difference between revisions of "IolE"

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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[iolA]]-[[iolB]]-[[iolC]]-[[iolD]]-[[iolE]]-[[iolF]]-[[iolG]]-[[iolH]]-[[iolJ]]''
+
* '''Operon:''' ''[[iolA]]-[[iolB]]-[[iolC]]-[[iolD]]-[[iolE]]-[[iolF]]-[[iolG]]-[[iolH]]-[[iolJ]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed]
  
* '''[[Sigma factor]]:''' [[SigA]]
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* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed]
  
* '''Regulation:''' repressed by glucose ([[CcpA]])
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* '''Regulation:'''  
 +
** repressed by glucose ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed]
 +
** induced by inositol ([[IolR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed]
  
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression  
+
* '''Regulatory mechanism:'''  
 +
** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed]
 +
** [[IolR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
* [[Yasutaro Fujita]], University of Fukuyama, Japan
 +
* [[Ken-ichi Yoshida]], Kobe University, Japan
  
 
=Your additional remarks=
 
=Your additional remarks=

Revision as of 17:29, 1 June 2009

  • Description: 2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)

Gene name iolE
Synonyms yxdE
Essential no
Product 2-keto-myo-inositol dehydratase,
dehydration of 2-keto-myo-inositol (2nd reaction)
Function myo-inositol catabolism
MW, pI 33 kDa, 5.209
Gene length, protein length 891 bp, 297 aa
Immediate neighbours iolF, iolD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
IolE context.gif
This image was kindly provided by SubtiList




The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2,4,6/3,5-pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O (according to Swiss-Prot)
  • Protein family: iolE/mocC family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Yoshida K, Yamaguchi M, Morinaga T, Kinehara M, Ikeuchi M, Ashida H, Fujita Y. (2008) myo-Inositol catabolism in Bacillus subtilis. J Biol Chem. Apr 18;283(16): 10415-24. PubMed
  2. Yoshida K., Yamaguchi M., Ikeda H., Omae K., Tsurusaki K., Fujita Y. (2004) The fifth gene of the iol operon of Bacillus subtilis, iolE, encodes 2-keto-myo-inositol dehydratase. Microbiology Mar;150(Pt 3): 571-80. PubMed