GlmM

• Description: phosphoglucosamine mutase, required for cell wall synthesis
  
  

Gene name	glmM
Synonyms	ybbT
Essential	yes PubMed
Product	phosphoglucosamine mutase, required for cell wall synthesis
Function	cell wall synthesis
Gene expression levels in SubtiExpress: glmM
Metabolic function and regulation of this protein in SubtiPathways: glmM
MW, pI	48 kDa, 4.677
Gene length, protein length	1344 bp, 448 aa
Immediate neighbours	cdaR, glmS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes, phosphoproteins

The gene

Basic information

• Locus tag: BSU01770

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU01770

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate (according to Swiss-Prot)

• Protein family: phosphohexose mutase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-100 PubMed, this is an autophosphorylation PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU01770

• Structure: 3PDK (from B. anthracis) PubMed

• UniProt: O34824

• KEGG entry: [2]

• E.C. number: 5.4.2.10

Expression and regulation

• Operon: cdaA-cdaR-glmM-glmS PubMed

• Expression browser: glmM PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between glmM and glmS PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1353 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2093 PubMed

Biological materials

• Mutant:

• Expression vector:
  • pGP400: (expression in B. subtilis in pBQ200), available in Jörg Stülke's lab
  • pGP1401: (expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • pGP1402: (cloning vector for glmM (GlmM S100A) in E. coli, in pBlueskript KS), available in Jörg Stülke's lab
  • pGP1403: (expression of GlmM (S100A) in B. subtilis in pBQ200), available in Jörg Stülke's lab
  • pGP1405: (expression, purification of GlmM (S100A) in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

• lacZ fusion:
  • GP1340 (cat) based on pAC6, available in Jörg Stülke's lab

• GFP fusion:
  • GP1382 glmM-gfp ermC (based on pGP1080), available in Jörg Stülke's lab

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• FLAG-tag construct:
  • GP1380 lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC (based on pGP1460 and pGP1087), available in Jörg Stülke's lab

• Strep-tag construct:
  • GP1388 lacA::glmM-Strep aphA3 cdaA-3xFLAG ermC (based on pGP1460 and pGP1087), available in Jörg Stülke's lab

• Antibody: available in Jörg Stülke's lab