ywlF

ywlF
168

ribose-5-phosphate isomerase

Locus
BSU_36920
Molecular weight
16.07 kDa
Isoelectric point
5.42
Protein length
Gene length
Function
pentose phosphate pathway
Product
ribose-5-phosphate isomerase
Essential
no
E.C.
5.3.1.-
Synonyms
ywlF, ipc-32d

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0698 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,791,199  3,791,648
Phenotypes of a mutant
poor growth PubMed
poorly transformable PubMed
The protein
Protein family
LacAB/RpiB family (single member, according to UniProt)
Structure
3HEE (PDB) (from Clostridium thermocellum, 55% identity, 72% similarity) PubMed
Effectors of protein activity
Inhibited by 6-phosphogluconate and AMP PubMed
Inhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ PubMed
Activated by EDTA and 2-mercaptoethanol PubMed
Kinetic information
Reversible Mass Action Kinetics PubMed PubMed
Additional information
The enzyme has probably more than one active site, but with no cooperativity described PubMed
Expression and Regulation
Operons
Genes
Description
Regulation
repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
Regulatory mechanism
TnrA: repression, PubMed, in tnrA regulon
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ywlFywlG

2025-05-02 19:01:26

ghost

77

62ada8abec2d5bd9e9db34bc019165f2f0c4db8a

469ED75E06AAD296F866D192899C88789118AFE5

Description
Open in new tab

ywlDywlG

2025-03-17 20:10:54

Jstuelk

130

78d3ea170d5a096ed935a0bae51120c6b96770c1

58F865E725DD290FE4DA109529748B35ABC8310F

Description
Open in new tab

ywlEywlG

2025-04-04 02:02:48

Jstuelk

99

4e928c421cc36003d43336f1b2a8ad6da5bb87e9

EE90CA1B239C34BDD25117ADD762430875D6B755

Biological materials
Mutant
BKE36920 (ywlF::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCTGCTTTTCCTCCCAGC,  downstream forward: _UP4_AAAAACCTGTAGGGGTGCTG
BKK36920 (ywlF::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCTGCTTTTCCTCCCAGC,  downstream forward: _UP4_AAAAACCTGTAGGGGTGCTG
FLAG-tag construct
GP1404 (spc, based on pGP1331), available in Jörg Stülke's lab
Antibody
**
References
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91827FEC70F5EEB8B072D06ABA05C19FC54D5F71

Page visits: 5238

Time of last update: 2025-05-02 00:23:57

Author of last update: Melvin.boenninger