Difference between revisions of "HemQ"

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(Biological materials)
(Biological materials)
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* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab  
 
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab  
 +
 +
* '''FLAG-tag vector:'''
 +
** pGP2658 ''hemQ-3xFLAG ermC'' (based on [[pBQ200]]), available in [[Jörg Stülke]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 11:40, 1 April 2015

  • Description: heme-binding protein, essential for heme biosynthesis

Gene name hemQ
Synonyms ipa-87r, ywfI
Essential no
Product heme-binding protein
Function biosynthesis of heme
Gene expression levels in SubtiExpress: hemQ
Metabolic function and regulation of this protein in SubtiPathways:
HemQ
MW, pI 29 kDa, 4.972
Gene length, protein length 762 bp, 254 aa
Immediate neighbours pta, bacG
Sequences Protein DNA DNA_with_flanks
Genetic context
YwfI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HemQ expression.png















Categories containing this gene/protein

biosynthesis of cofactors, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU37670

Phenotypes of a mutant

defect in heme biosynthesis

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0447 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-41 PubMed
  • Cofactor(s): heme
  • Effectors of protein activity:

Database entries

  • Structure: 1T0T (Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 358 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1041 PubMed

Biological materials

  • Mutant:
  • Expression vector:
    • pGP2643: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
    • pGP2644: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References