Difference between revisions of "BcaP"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' BKE09460 (''[[bcaP]]''::''erm'', available in the BGSC and in [[Fabian Commichau]]'s lab)
+
* '''Mutant:''' BKE09460 (''[[bcaP]]''::''erm'', available in the BGSC and in [[Fabian Commichau]]'s and [[Jörg Stülke]]'s labs)
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 15:01, 4 February 2015

  • Description: branched-chain amino acid transporter, major transporter for isoleucine and valine, may also be involved in threonine transport

Gene name bcaP
Synonyms yhdG
Essential no
Product branched-chain amino acid transporter
Function biosynthesis/acquisition of branched-chain amino acids
Gene expression levels in SubtiExpress: bcaP
Metabolic function and regulation of this protein in SubtiPathways:
BcaP
MW, pI 49 kDa, 9.735
Gene length, protein length 1395 bp, 465 aa
Immediate neighbours yhdF, yhdH
Sequences Protein DNA DNA_with_flanks
Genetic context
YhdG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BcaP expression.png















Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

  • Locus tag: BSU09460

Phenotypes of a mutant

  • no phenotype for the single mutant, the triple bcaP brnQ braB mutant is strongly impaired in the transport of isoleucine and valine at low concentrations PubMed
  • resistant to growth inhibition by high concentrations of threonine PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • high affinity uptake of isoleucine and valine PubMed
  • Paralogous protein(s): YfnA

Extended information on the protein

  • Kinetic information:
    • for Ile: Km ≈ 4.1 μM; Vmax at 26°C was ~15 nmol/min/ mg protein PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Boris R Belitsky
Role of branched-chain amino acid transport in Bacillus subtilis CodY activity.
J Bacteriol: 2015, 197(8);1330-8
[PubMed:25645558] [WorldCat.org] [DOI] (I p)

Boris R Belitsky, Abraham L Sonenshein
Roadblock repression of transcription by Bacillus subtilis CodY.
J Mol Biol: 2011, 411(4);729-43
[PubMed:21699902] [WorldCat.org] [DOI] (I p)

Boris R Belitsky, Abraham L Sonenshein
Contributions of multiple binding sites and effector-independent binding to CodY-mediated regulation in Bacillus subtilis.
J Bacteriol: 2011, 193(2);473-84
[PubMed:21097623] [WorldCat.org] [DOI] (I p)

Shaun R Brinsmade, Roelco J Kleijn, Uwe Sauer, Abraham L Sonenshein
Regulation of CodY activity through modulation of intracellular branched-chain amino acid pools.
J Bacteriol: 2010, 192(24);6357-68
[PubMed:20935095] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Boris R Belitsky, Abraham L Sonenshein
Genetic and biochemical analysis of CodY-binding sites in Bacillus subtilis.
J Bacteriol: 2008, 190(4);1224-36
[PubMed:18083814] [WorldCat.org] [DOI] (I p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)