Difference between revisions of "BcaP"

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(Phenotypes of a mutant)
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
* no phenotype for the single mutant, the triple ''[[bcaP]] [[brnQ]] [[braB]]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations {{PubMed|25645558}}
 
* no phenotype for the single mutant, the triple ''[[bcaP]] [[brnQ]] [[braB]]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations {{PubMed|25645558}}
 +
* resistant to growth inhibition by high concentrations of threonine {{PubMed|25645558}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 14:56, 4 February 2015

  • Description: branched-chain amino acid transporter, major transporter for isoleucine and valine

Gene name bcaP
Synonyms yhdG
Essential no
Product branched-chain amino acid transporter
Function biosynthesis/acquisition of branched-chain amino acids
Gene expression levels in SubtiExpress: bcaP
Metabolic function and regulation of this protein in SubtiPathways:
BcaP
MW, pI 49 kDa, 9.735
Gene length, protein length 1395 bp, 465 aa
Immediate neighbours yhdF, yhdH
Sequences Protein DNA DNA_with_flanks
Genetic context
YhdG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BcaP expression.png















Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

  • Locus tag: BSU09460

Phenotypes of a mutant

  • no phenotype for the single mutant, the triple bcaP brnQ braB mutant is strongly impaired in the transport of isoleucine and valine at low concentrations PubMed
  • resistant to growth inhibition by high concentrations of threonine PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • high affinity uptake of isoleucine and valine PubMed
  • Paralogous protein(s): YfnA

Extended information on the protein

  • Kinetic information:
    • for Ile: Km ≈ 4.1 μM; Vmax at 26°C was ~15 nmol/min/ mg protein PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References