Difference between revisions of "BraB"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
{{SubtiWiki category|[[transporters/ other]]}}, | {{SubtiWiki category|[[transporters/ other]]}}, | ||
{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}}, | {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}}, | ||
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{{SubtiWiki category|[[membrane proteins]]}} | {{SubtiWiki category|[[membrane proteins]]}} | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * no phenotype for the single mutant, the triple ''[[bcaP]] [[brnQ]] [[braB]]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations {{PubMed|25645558}} | ||
=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| + | ** high affinity uptake of isoleucine and valine {{PubMed|25645558}} | ||
* '''Protein family:''' branched chain amino acid transporter family (according to Swiss-Prot) | * '''Protein family:''' branched chain amino acid transporter family (according to Swiss-Prot) | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| + | ** for Ile: Km ≈ 16 μM and Vmax ≈ 25 nmol/min/ mg protein at 37°C {{PubMed|25645558}} | ||
* '''Domains:''' | * '''Domains:''' | ||
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=References= | =References= | ||
| − | <pubmed> 9387221 </pubmed> | + | <pubmed> 9387221 25645558</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:47, 4 February 2015
- Description: branched-chain amino acid transporter
| Gene name | braB |
| Synonyms | |
| Essential | no |
| Product | branched-chain amino acid transporter |
| Function | uptake of branched-chain amino acids |
| Gene expression levels in SubtiExpress: braB | |
| Metabolic function and regulation of this protein in SubtiPathways: braB | |
| MW, pI | 46 kDa, 9.65 |
| Gene length, protein length | 1335 bp, 445 aa |
| Immediate neighbours | nifZ, ezrA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
transporters/ other, biosynthesis/ acquisition of amino acids, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29600
Phenotypes of a mutant
- no phenotype for the single mutant, the triple bcaP brnQ braB mutant is strongly impaired in the transport of isoleucine and valine at low concentrations PubMed
Database entries
- BsubCyc: BSU29600
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- high affinity uptake of isoleucine and valine PubMed
- Protein family: branched chain amino acid transporter family (according to Swiss-Prot)
- Paralogous protein(s): BrnQ
Extended information on the protein
- Kinetic information:
- for Ile: Km ≈ 16 μM and Vmax ≈ 25 nmol/min/ mg protein at 37°C PubMed
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU29600
- Structure:
- UniProt: O34545
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Boris R Belitsky
Role of branched-chain amino acid transport in Bacillus subtilis CodY activity.
J Bacteriol: 2015, 197(8);1330-8
[PubMed:25645558]
[WorldCat.org]
[DOI]
(I p)
Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221]
[WorldCat.org]
[DOI]
(P p)
