SpoIIIJ

• Description: Sec-independent membrane protein translocase, essential for SigG activity at stage III, involved in the assembly of the SpoIIIA-SpoIIQ complex
  
  

Gene name	spoIIIJ
Synonyms	spo0J87
Essential	no
Product	membrane protein translocase
Function	membrane insertion of proteins and protein secretion
Gene expression levels in SubtiExpress: spoIIIJ
Interactions involving this protein in SubtInteract: SpoIIIJ
MW, pI	29 kDa, 9.992
Gene length, protein length	783 bp, 261 aa
Immediate neighbours	jag, rnpA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein secretion, sporulation/ other, membrane proteins

The gene

Basic information

• Locus tag: BSU41040

Database entries

• BsubCyc: BSU41040

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: membrane protein translocase, facilitates insertion of SpoIIIAE into the membrane PubMed

• Protein family: YidC/Oxa1/Alb3 family PubMed

• Paralogous protein(s): YidC2, one of the two proteins has to be present for viability PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • the active form is a dimer PubMed
  • SpoIIIAE-SpoIIIJ PubMed
  • SpoIIIJ-MifM PubMed

• Localization:
  • membrane PubMed

Database entries

• BsubCyc: BSU41040

• Structure:

• UniProt: Q01625

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIIJ-jag PubMed

• Expression browser: spoIIIJ PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1044 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard University, Cambridge, USA homepage

Adriano Henriques, Lisbon, Portugal homepage