Difference between revisions of "SigL"
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* '''Mutant:''' | * '''Mutant:''' | ||
− | ** GP146 ( ''sigL'':: | + | ** GP146 ( ''sigL''::spc), available in [[Jörg Stülke]]'s lab {{PubMed|17183217}} |
** 1A914 ( ''sigL''::''kan''), {{PubMed|16585774}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A914&Search=1A914 BGSC] | ** 1A914 ( ''sigL''::''kan''), {{PubMed|16585774}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A914&Search=1A914 BGSC] | ||
Revision as of 12:31, 23 July 2014
- Description: sigma factor of the RNA polymerase, Sigma-54, Sigma L
Gene name | sigL |
Synonyms | |
Essential | no |
Product | RNA polymerase sigma-54 factor (sigma-L) |
Function | utilization of arginin, acetoin and fructose, required for cold adaptation |
Gene expression levels in SubtiExpress: sigL | |
Interactions involving this protein in SubtInteract: SigL | |
MW, pI | 49,5 kDa, 7.79 |
Gene length, protein length | 1308 bp, 436 amino acids |
Immediate neighbours | yvfH, yvfG |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
transcription, sigma factors and their control
This gene is a member of the following regulons
The SigL regulon
The gene
Basic information
- Locus tag: BSU34200
Phenotypes of a mutant
The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed
Database entries
- BsubCyc: BSU34200
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type
- Protein family: sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family
- Paralogous protein(s):
Transcription factors activating transcription at SigL-dependent promoters
Extended information on the protein
- Kinetic information:
- Domains:
- DNA binding domain (H-T-H motif) (324–343)
- pron box domain (413–421)
- 3 x Compositional bias domain (6–21),(32–53),(112–136)
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU34200
- Structure:
- UniProt: P24219
- KEGG entry: [3]
Additional information
Transcription initiation by SigL-containing RNA polymerase requires the activity of ATP-hydrolyzing transcription activators.
Transcription factors activating transcription at SigL-dependent promoters
Expression and regulation
- Operon: sigL (according to DBTBS)
- Additional information:
Biological materials
- Mutant:
- GP146 ( sigL::spc), available in Jörg Stülke's lab PubMed
- 1A914 ( sigL::kan), PubMed, available at BGSC
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
Your additional remarks
References
Reviews
Victoria Shingler
Signal sensory systems that impact σ⁵⁴ -dependent transcription.
FEMS Microbiol Rev: 2011, 35(3);425-40
[PubMed:21054445]
[WorldCat.org]
[DOI]
(I p)
M Buck, M T Gallegos, D J Studholme, Y Guo, J D Gralla
The bacterial enhancer-dependent sigma(54) (sigma(N)) transcription factor.
J Bacteriol: 2000, 182(15);4129-36
[PubMed:10894718]
[WorldCat.org]
[DOI]
(P p)
M J Merrick
In a class of its own--the RNA polymerase sigma factor sigma 54 (sigma N).
Mol Microbiol: 1993, 10(5);903-9
[PubMed:7934866]
[WorldCat.org]
[DOI]
(P p)
Original publications
Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538]
[WorldCat.org]
[DOI]
(I e)
Frank Wiegeshoff, Carsten L Beckering, Michel Debarbouille, Mohamed A Marahiel
Sigma L is important for cold shock adaptation of Bacillus subtilis.
J Bacteriol: 2006, 188(8);3130-3
[PubMed:16585774]
[WorldCat.org]
[DOI]
(P p)
Soo-Keun Choi, Milton H Saier
Regulation of sigL expression by the catabolite control protein CcpA involves a roadblock mechanism in Bacillus subtilis: potential connection between carbon and nitrogen metabolism.
J Bacteriol: 2005, 187(19);6856-61
[PubMed:16166551]
[WorldCat.org]
[DOI]
(P p)
N O Ali, J Bignon, G Rapoport, M Debarbouille
Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis.
J Bacteriol: 2001, 183(8);2497-504
[PubMed:11274109]
[WorldCat.org]
[DOI]
(P p)
M Débarbouillé, I Martin-Verstraete, F Kunst, G Rapoport
The Bacillus subtilis sigL gene encodes an equivalent of sigma 54 from gram-negative bacteria.
Proc Natl Acad Sci U S A: 1991, 88(20);9092-6
[PubMed:1924373]
[WorldCat.org]
[DOI]
(P p)