Difference between revisions of "Spo0A"

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(Reviews)
(Basic information/ Evolution)
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
 
** activation of gene expression at the onset of [[sporulation]] ([[Spo0A regulon]])
 
** activation of gene expression at the onset of [[sporulation]] ([[Spo0A regulon]])
** coordination between DNA replication and initiation of [[sporulation]] by binding to sites close to the ''oriC'' (this limits re-initiation of replication) {{PubMed|23301687}}
+
** coordination between [[DNA replication]] and initiation of [[sporulation]] by binding to sites close to the ''oriC'' (this limits re-initiation of replication) {{PubMed|23301687}}
  
 
* '''Protein family:'''
 
* '''Protein family:'''

Revision as of 07:53, 11 June 2014

Gene name spo0A
Synonyms spo0C, spo0G, spoIIL, sof-1
Essential no
Product phosphorelay response regulator
Function initiation of sporulation
Gene expression levels in SubtiExpress: spo0A
Interactions involving this protein in SubtInteract: Spo0A
Metabolic function and regulation of this protein in SubtiPathways:
spo0A
MW, pI 29 kDa, 5.989
Gene length, protein length 801 bp, 267 aa
Immediate neighbours yqiG, spoIVB
Sequences Protein DNA DNA_with_flanks
Genetic context
Spo0A context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Spo0A expression.png















Categories containing this gene/protein

transcription factors and their control, phosphorelay, biofilm formation, phosphoproteins

This gene is a member of the following regulons

SigH regulon, Spo0A regulon

The Spo0A regulon

The gene

Basic information

  • Locus tag: BSU24220

Phenotypes of a mutant

  • inactivation of spo0A restores beta-lactam resistance in a sigM mutant PubMed
  • altered cell death pattern in colonies PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: receives phosphorylation from Spo0B, dephosphorylation by Spo0E, direct phosphorylation by KinC upon potassium leakage PubMed
  • Cofactor(s):
  • Effectors of protein activity:
    • the phosphorylation state affects DNA binding activity
    • interaction with MecA inhibits the transcriptional activity of Spo0A∼P PubMed

Database entries

  • Structure: 1QMP (with phosphorylated aspartate, Geobacillus stearothermophilus), 1FC3 (trans-activation domain, Geobacillus stearothermophilus), 1LQ1 (complex with DNA)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed by casamino acids PubMed
    • repressed by SinR overexpression PubMed
    • repressed by soj PubMed
    • expression increases when drowth rate declines PubMed
  • Regulatory mechanism:
    • Spo0A: transcription repression PubMed
    • Spo0A synthesis is subject to a post-transcriptional control mechanism such that translation of mRNAs originating from the SigA-dependent promoter is impeded due to RNA secondary structure PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 394 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 358 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1763 PubMed

Biological materials

  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


The Spo0A regulon

Other original Publications