LplJ

• Description: lipoate-protein ligase
  
  

Gene name	lplJ
Synonyms	yhfJ
Essential	no
Product	Lipoate:protein ligase
Function	lipoic acid metabolism
Gene expression levels in SubtiExpress: lplJ
Metabolic function and regulation of this protein in SubtiPathways: LplJ
MW, pI	37 kDa, 5.835
Gene length, protein length	993 bp, 331 aa
Immediate neighbours	yhfI, yhfK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU10250

Database entries

• BsubCyc: BSU10250

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU10250

• Structure:

• UniProt: O07608

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: lplJ PubMed

• Sigma factor:

• Regulation: induced by valine PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 150 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 287 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Quin H Christensen, Natalia Martin, Maria C Mansilla, Diego de Mendoza, John E Cronan
A novel amidotransferase required for lipoic acid cofactor assembly in Bacillus subtilis.
Mol Microbiol: 2011, 80(2);350-63
[PubMed:21338421] [WorldCat.org] [DOI] (I p)

Natalia Martin, Quin H Christensen, María C Mansilla, John E Cronan, Diego de Mendoza
A novel two-gene requirement for the octanoyltransfer reaction of Bacillus subtilis lipoic acid biosynthesis.
Mol Microbiol: 2011, 80(2);335-49
[PubMed:21338420] [WorldCat.org] [DOI] (I p)