Difference between revisions of "ComK"

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* '''Additional information:'''
 
* '''Additional information:'''
 
** degraded by [[ClpC]]-[[ClpP]] (with [[MecA]] as adaptor protein) {{PubMed|9890793}}
 
** degraded by [[ClpC]]-[[ClpP]] (with [[MecA]] as adaptor protein) {{PubMed|9890793}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 49 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:57, 17 April 2014

  • Description: competence transcription factor (CTF)

Gene name comK
Synonyms
Essential no
Product competence transcription factor (CTF)
Function regulation of competence and DNA uptake
Gene expression levels in SubtiExpress: comK
Interactions involving this protein in SubtInteract: ComK
Metabolic function and regulation of this protein in SubtiPathways:
comK
MW, pI 22 kDa, 7.669
Gene length, protein length 576 bp, 192 aa
Immediate neighbours yhzC, yhxD
Sequences Protein DNA DNA_with_flanks
Genetic context
ComK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ComK expression.png















Categories containing this gene/protein

genetic competence, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, CodY regulon, ComK regulon, DegU regulon, Rok regulon, Spo0A regulon

The ComK regulon

The gene

Basic information

  • Locus tag: BSU10420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated on Arg-65, Arg-157, Arg-161, Arg-165, Arg-186, and Arg-191 PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed by Rok PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • repressed during logrithmic growth (AbrB) PubMed
    • induced at the entry to stationary phase (Spo0A-P) PubMed
  • Regulatory mechanism:
  • Additional information:
    • degraded by ClpC-ClpP (with MecA as adaptor protein) PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 49 PubMed

Biological materials

  • Mutant:
  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Oscar Kuipers, University of Groningen, The Netherlands Homepage

Your additional remarks

References

Reviews

Daniel Schultz, Peter G Wolynes, Eshel Ben Jacob, José N Onuchic
Deciding fate in adverse times: sporulation and competence in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2009, 106(50);21027-34
[PubMed:19995980] [WorldCat.org] [DOI] (I p)

Leendert W Hamoen, Gerard Venema, Oscar P Kuipers
Controlling competence in Bacillus subtilis: shared use of regulators.
Microbiology (Reading): 2003, 149(Pt 1);9-17
[PubMed:12576575] [WorldCat.org] [DOI] (P p)

The ComK regulon

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)

Randy M Berka, Jeanette Hahn, Mark Albano, Irena Draskovic, Marjan Persuh, Xianju Cui, Alan Sloma, William Widner, David Dubnau
Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK.
Mol Microbiol: 2002, 43(5);1331-45
[PubMed:11918817] [WorldCat.org] [DOI] (P p)

Original Publications