NadE

• Description: NH3-dependent NAD+ synthetase
  
  

Gene name	nadE
Synonyms	outB, tscBGH, gsp-81
Essential	yes PubMed
Product	NH3-dependent NAD+ synthetase
Function	NAD biosynthesis
Gene expression levels in SubtiExpress: nadE
Metabolic function and regulation of this protein in SubtiPathways: NadE
MW, pI	30 kDa, 4.888
Gene length, protein length	816 bp, 272 aa
Immediate neighbours	ycgI, tmrB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, general stress proteins (controlled by SigB), essential genes

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU03130

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU03130

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + deamido-NAD⁺ + NH₃ = AMP + diphosphate + NAD⁺ (according to Swiss-Prot)

• Protein family: NAD synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU03130

• Structure: 1KQP

• UniProt: P08164

• KEGG entry: [3]

• E.C. number: 6.3.1.5

Expression and regulation

• Operon: nadE PubMed

• Expression browser: nadE PubMed

• Sigma factor: SigA, SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed
  • induced by antibiotics interacting with the cytoplasmic membrane PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 684 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 6179 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: