YjiB

• Description: cytochrome P450, monooxygenase CYP109B1
  
  

Gene name	yjiB
Synonyms
Essential	no
Product	monooxygenase CYP109B1
Function	oxidation of fatty acids
Gene expression levels in SubtiExpress: yjiB
Interactions involving this protein in SubtInteract: YjiB
Metabolic function and regulation of this protein in SubtiPathways: YjiB
MW, pI	44 kDa, 5.379
Gene length, protein length	1188 bp, 396 aa
Immediate neighbours	yjiA, yjiC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, lipid metabolism/ other

The gene

Basic information

• Locus tag: BSU12210

Database entries

• BsubCyc: BSU12210

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • oxidation of saturated fatty acids (conversion up to 99%) and their methyl and ethyl esters (conversion up to 80%) at subterminal positions with a preference for the carbon atoms C11 and C12 counted from the carboxyl group PubMed

• Protein family: cytochrome P450 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YjiB-YkuN PubMed, YjiB-YkuP PubMed

• Localization:

Database entries

• BsubCyc: BSU12210

• Structure:

• UniProt: O34374

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yjiB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Marco Girhard, Tobias Klaus, Yogan Khatri, Rita Bernhardt, Vlada B Urlacher
Characterization of the versatile monooxygenase CYP109B1 from Bacillus subtilis.
Appl Microbiol Biotechnol: 2010, 87(2);595-607
[PubMed:20186410] [WorldCat.org] [DOI] (I p)

Marco Girhard, Kazuhiro Machida, Masashi Itoh, Rolf D Schmid, Akira Arisawa, Vlada B Urlacher
Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system.
Microb Cell Fact: 2009, 8;36
[PubMed:19591681] [WorldCat.org] [DOI] (I e)