IspG

• Description: similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase, 6th step in the MEP pathway of isoprenoid biosynthesis
  
  

Gene name	ispG
Synonyms	yqfY
Essential	yes PubMed
Product	1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase
Function	MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: ispG
Metabolic function and regulation of this protein in SubtiPathways: IspG
MW, pI	40 kDa, 6.802
Gene length, protein length	1131 bp, 377 aa
Immediate neighbours	yqfZ, yqfX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, sporulation proteins, essential genes

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU25070

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU25070

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H₂O + protein-disulfide = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + protein-dithiol (according to Swiss-Prot)

• Protein family: ispG family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): contains an iron-sulfur cluster

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• BsubCyc: BSU25070

• Structure: 3NOY (from Aquifex aeolicus, 51% identity) PubMed

• UniProt: P54482

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yqfZ-yqfY PubMed

• Expression browser: ispG PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Becky M Hess, Junfeng Xue, Lye Meng Markillie, Ronald C Taylor, H Steven Wiley, Birgitte K Ahring, Bryan Linggi
Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics.
PLoS One: 2013, 8(6);e66104
[PubMed:23840410] [WorldCat.org] [DOI] (I e)

Kazuya Nakagawa, Kentaro Takada, Nobutaka Imamura
Probable novel MEP pathway inhibitor and its binding protein, IspG.
Biosci Biotechnol Biochem: 2013, 77(7);1449-54
[PubMed:23832336] [WorldCat.org] [DOI] (I p)

Matthias Lee, Tobias Gräwert, Felix Quitterer, Felix Rohdich, Jörg Eppinger, Wolfgang Eisenreich, Adelbert Bacher, Michael Groll
Biosynthesis of isoprenoids: crystal structure of the [4Fe-4S] cluster protein IspG.
J Mol Biol: 2010, 404(4);600-10
[PubMed:20932974] [WorldCat.org] [DOI] (I p)

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)