Difference between revisions of "RocR"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40350&redirect=T BSU40350] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rocR.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rocR.html] | ||
| Line 98: | Line 99: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40350&redirect=T BSU40350] | ||
* '''Structure:''' | * '''Structure:''' | ||
Revision as of 15:16, 2 April 2014
- Description: transcriptional activator of arginine utilization operons
| Gene name | rocR |
| Synonyms | |
| Essential | no |
| Product | transcriptional activator |
| Function | transcriptional activator of arginine utilization operons |
| Gene expression levels in SubtiExpress: rocR | |
| Interactions involving this protein in SubtInteract: RocR | |
| Metabolic function and regulation of this protein in SubtiPathways: rocR | |
| MW, pI | 52.6 kDa, 6.08 |
| Gene length, protein length | 1383 bp, 461 amino acids |
| Immediate neighbours | rocD, yyzO |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
transcription factors and their control
- see also: glutamate metabolism
This gene is a member of the following regulons
The RocR regulon
The gene
Basic information
- Locus tag: BSU40350
Phenotypes of a mutant
Database entries
- BsubCyc: BSU40350
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transcription activation at SigL-dependent promoters of rocABC, rocDEF, and rocG
- Protein family: Sigma-54 interacting transcription activator
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Sigma-54 factor interaction domain (143–372)
- 2x nucleotid-binding domain (171–178),(233–242)
- HTH motif (434–453)
- Modification:
- Cofactor(s): ornithine or citrulline are required for RocR-dependent transcription activation PubMed
- Effectors of protein activity:
- Interactions:
- RocR-SigL
Database entries
- BsubCyc: BSU40350
- Structure:
- UniProt: P38022
- KEGG entry: [3]
Additional information
Expression and regulation
- Operon: rocR (according to DBTBS)
- Regulatory mechanism: autorepression
- Additional information:
Biological materials
- Mutant: QB5533 (aphA3), from Michel Debarbouille, available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
Your additional remarks
References
Adrienne Zaprasis, Tamara Hoffmann, Guido Wünsche, Lope A Flórez, Jörg Stülke, Erhard Bremer
Mutational activation of the RocR activator and of a cryptic rocDEF promoter bypass loss of the initial steps of proline biosynthesis in Bacillus subtilis.
Environ Microbiol: 2014, 16(3);701-17
[PubMed:23869754]
[WorldCat.org]
[DOI]
(I p)
Kenji Manabe, Yasushi Kageyama, Takuya Morimoto, Tadahiro Ozawa, Kazuhisa Sawada, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara, Katsuya Ozaki, Naotake Ogasawara
Combined effect of improved cell yield and increased specific productivity enhances recombinant enzyme production in genome-reduced Bacillus subtilis strain MGB874.
Appl Environ Microbiol: 2011, 77(23);8370-81
[PubMed:21965396]
[WorldCat.org]
[DOI]
(I p)
Naima Ould Ali, Josette Jeusset, Eric Larquet, Eric Le Cam, Boris Belitsky, Abraham L Sonenshein, Tarek Msadek, Michel Débarbouillé
Specificity of the interaction of RocR with the rocG-rocA intergenic region in Bacillus subtilis.
Microbiology (Reading): 2003, 149(Pt 3);739-750
[PubMed:12634342]
[WorldCat.org]
[DOI]
(P p)
R Gardan, G Rapoport, M Débarbouillé
Role of the transcriptional activator RocR in the arginine-degradation pathway of Bacillus subtilis.
Mol Microbiol: 1997, 24(4);825-37
[PubMed:9194709]
[WorldCat.org]
[DOI]
(P p)
S Calogero, R Gardan, P Glaser, J Schweizer, G Rapoport, M Debarbouille
RocR, a novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators.
J Bacteriol: 1994, 176(5);1234-41
[PubMed:8113162]
[WorldCat.org]
[DOI]
(P p)
