Difference between revisions of "PepT"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU38920&redirect=T BSU38920]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pepT.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pepT.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU38920&redirect=T BSU38920]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3GB0 3GBO] (from ''Bacillus cereus (71% identity''), to be published)
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3GB0 3GBO] (from ''Bacillus cereus (71% identity''), to be published)

Revision as of 15:10, 2 April 2014

  • Description: peptidase T (tripeptidase), zinc-dependent

Gene name pepT
Synonyms
Essential no
Product peptidase T (tripeptidase)
Function peptide degradation
Gene expression levels in SubtiExpress: pepT
Metabolic function and regulation of this protein in SubtiPathways:
pepT
MW, pI 45 kDa, 4.538
Gene length, protein length 1230 bp, 410 aa
Immediate neighbours yxjL, yxjJ
Sequences Protein DNA DNA_with_flanks
Genetic context
PepT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PepT expression.png
























Categories containing this gene/protein

utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU38920

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Release of the N-terminal residue from a tripeptide (according to Swiss-Prot)
  • Protein family: peptidase M20B family (according to Swiss-Prot)
  • Paralogous protein(s): YqjE

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3GBO (from Bacillus cereus (71% identity), to be published)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

M H Cha, W M Yong, S M Lee, Y S Lee, I Y Chung
The biochemical and molecular characterization of recombinant Bacillus subtilis tripeptidase (PepT) as a zinc-dependent metalloenzyme.
Mol Cells: 2000, 10(4);423-31
[PubMed:10987140] [WorldCat.org] (P p)

O Schrögel, O Krispin, R Allmansberger
Expression of a pepT homologue from Bacillus subtilis.
FEMS Microbiol Lett: 1996, 145(3);341-8
[PubMed:8978088] [WorldCat.org] [DOI] (P p)