Difference between revisions of "ArgG"

From SubtiWiki
Jump to: navigation, search
Line 56: Line 56:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29450&redirect=T BSU29450]
  
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
Line 92: Line 93:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29450&redirect=T BSU29450]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1KH1 1KH1] (from ''Thermus thermophilus'', 44% identity, 65% similarity) {{PubMed|11844799}}
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1KH1 1KH1] (from ''Thermus thermophilus'', 44% identity, 65% similarity) {{PubMed|11844799}}

Revision as of 14:30, 2 April 2014

  • Description: argininosuccinate synthase, reversible

Gene name argG
Synonyms
Essential no
Product argininosuccinate synthase, reversible
Function biosynthesis of arginine
Gene expression levels in SubtiExpress: argG
Metabolic function and regulation of this protein in SubtiPathways:
argG
MW, pI 44 kDa, 5.028
Gene length, protein length 1209 bp, 403 aa
Immediate neighbours argH, moaB
Sequences Protein DNA DNA_with_flanks
Genetic context
ArgG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
500px















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

AhrC regulon

The gene

Basic information

  • Locus tag: BSU29450

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate (according to Swiss-Prot)
  • Protein family: Type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • S-cysteinylation after diamide stress (C187) PubMed
    • phosphorylated on Arg-262 PubMed
  • Effectors of protein activity:

Database entries

  • Structure: 1KH1 (from Thermus thermophilus, 44% identity, 65% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed by arginine (AhrC)
  • Regulatory mechanism:
    • AhrC: transcription repression

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References