SpoVM

• Description: required for normal spore cortex and coat synthesis, inhibits the proteolytic activity of FtsH (adaptor protein)
  
  

Gene name	spoVM
Synonyms
Essential	no
Product	spore coat morphogenetic protein
Function	spore cortex and coat synthesis
Gene expression levels in SubtiExpress: spoVM
Interactions involving this protein in SubtInteract: SpoVM
MW, pI	2 kDa, 11.026
Gene length, protein length	78 bp, 26 aa
Immediate neighbours	yloS, rpmB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU15810

Phenotypes of a mutant

• reduction of sporulation efficiency due to defects in spore coat and cortex assembly PubMed

Database entries

• BsubCyc: BSU15810

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • anchors the basement layer of the spore coat to the surface of the developing spore PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: contains an amphipathic alpha-helix

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • SpoIVA-SpoVM-SpoVID PubMed
  • SpoVM-FtsH PubMed

• Localization:
  • around the surface of the developing forespore PubMed
  • spore membrane, recognizes membrane curvature PubMed
  • spore coat (basement), localization depends on SpoIVA PubMed

Database entries

• BsubCyc: BSU15810

• Structure:

• UniProt: P37817

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoVM PubMed

• Expression browser: spoVM PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed in mother cell during sporulation (SigE, SpoIIID) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Ana Yepes, Johannes Schneider, Benjamin Mielich, Gudrun Koch, Juan-Carlos García-Betancur, Kumaran S Ramamurthi, Hera Vlamakis, Daniel López
The biofilm formation defect of a Bacillus subtilis flotillin-defective mutant involves the protease FtsH.
Mol Microbiol: 2012, 86(2);457-71
[PubMed:22882210] [WorldCat.org] [DOI] (I p)

Sarah E Ebmeier, Irene S Tan, Katie Rose Clapham, Kumaran S Ramamurthi
Small proteins link coat and cortex assembly during sporulation in Bacillus subtilis.
Mol Microbiol: 2012, 84(4);682-96
[PubMed:22463703] [WorldCat.org] [DOI] (I p)

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Katherine H Wang, Anabela L Isidro, Lia Domingues, Haig A Eskandarian, Peter T McKenney, Kevin Drew, Paul Grabowski, Ming-Hsiu Chua, Samantha N Barry, Michelle Guan, Richard Bonneau, Adriano O Henriques, Patrick Eichenberger
The coat morphogenetic protein SpoVID is necessary for spore encasement in Bacillus subtilis.
Mol Microbiol: 2009, 74(3);634-49
[PubMed:19775244] [WorldCat.org] [DOI] (I p)

Kumaran S Ramamurthi, Sigolene Lecuyer, Howard A Stone, Richard Losick
Geometric cue for protein localization in a bacterium.
Science: 2009, 323(5919);1354-7
[PubMed:19265022] [WorldCat.org] [DOI] (I p)

Ai Thi Thuy Le, Wolfgang Schumann
Regulation of the spoVM gene of Bacillus subtilis.
Curr Microbiol: 2008, 57(5);484-9
[PubMed:18820968] [WorldCat.org] [DOI] (P p)

Kumaran S Ramamurthi, Katie Rose Clapham, Richard Losick
Peptide anchoring spore coat assembly to the outer forespore membrane in Bacillus subtilis.
Mol Microbiol: 2006, 62(6);1547-57
[PubMed:17427285] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Christiaan van Ooij, Richard Losick
Subcellular localization of a small sporulation protein in Bacillus subtilis.
J Bacteriol: 2003, 185(4);1391-8
[PubMed:12562810] [WorldCat.org] [DOI] (P p)

R S Prajapati, T Ogura, S M Cutting
Structural and functional studies on an FtsH inhibitor from Bacillus subtilis.
Biochim Biophys Acta: 2000, 1475(3);353-9
[PubMed:10913836] [WorldCat.org] [DOI] (P p)

K D Price, R Losick
A four-dimensional view of assembly of a morphogenetic protein during sporulation in Bacillus subtilis.
J Bacteriol: 1999, 181(3);781-90
[PubMed:9922240] [WorldCat.org] [DOI] (P p)

S Cutting, M Anderson, E Lysenko, A Page, T Tomoyasu, K Tatematsu, T Tatsuta, L Kroos, T Ogura
SpoVM, a small protein essential to development in Bacillus subtilis, interacts with the ATP-dependent protease FtsH.
J Bacteriol: 1997, 179(17);5534-42
[PubMed:9287010] [WorldCat.org] [DOI] (P p)

P A Levin, N Fan, E Ricca, A Driks, R Losick, S Cutting
An unusually small gene required for sporulation by Bacillus subtilis.
Mol Microbiol: 1993, 9(4);761-71
[PubMed:8231808] [WorldCat.org] [DOI] (P p)